Microarray technology in practice
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Steven Russell; Lisa A Meadows; Roslin R Russell
- ناشر : Amsterdam : Elsevier Academic Press
- چاپ و سال / کشور: 2009
- شابک / ISBN : 9780123725165
Description
Foreword and acknowledgments ix 1. Introduction 1.1. Technology 1 1.2. A Brief History 4 1.3. A Brief Outline 9 References 13 2 The Basics of Experimental Design 2.1. Sources of Variation in Microarray Gene Expression Measurements 18 2.2. Controls and Replicates 21 2.3. Experimental Designs 26 2.4. Summary 32 References 32 3 Designing and Producing Microarrays 3.1. Probe Selection 36 3.2. cDNA and Amplicon Probes 39 3.3. Oligonucleotide Probes 40 3.4. Preparing Arrays 55 3.5. Summary 65 References 65 4 Sample Collection and Labeling 4.1. Sample Collection and RNA Extraction 72 4.2. RNA Quality Assessment 73 4.3. cDNA Production 76 4.4. Labeling Methods 78 4.5. Signal Amplifi cation 84 v
As with other large scale genomics approaches, microarray technologies are broadly applicable across disciplines of life and biomedical sciences, but remain daunting to many researchers. This guide is designed to demystify the technology and inform more biologists about this critically important experimental technique
