MRNA formation and function
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Joel D Richter
- ناشر : San Diego : Academic Press
- چاپ و سال / کشور: 1997
- شابک / ISBN : 9780125875455
Description
The Tat-TAR RNP, a master switch that regulates HIV-1 gene expression--HeLa nuclear extract: a modified protocol--Functional analysis of splicing factors and regulators--Identification of pre-mRNA splicing factors and analysis of RNA-protein interaction--In Vitro analysis of mammalian cell mRNA 3' processing--Rapid identification and cloning of sequence-specific RNA binding proteins--Analysis of polyadenylation phenotypes in Saccharomyces cerevisiae--Poly(A) polymerase/Cap-specific 2'-O-methyltransferase from Vaccinia virus: expression, purification, uses, and protein-ligand interaction assays--A genetic approach to mapping coding region determinants of mRNA instability in yeast--Identification of the protein that interacts with the 3' end of histone mRNA--Analysis of mRNP complexes assembled in Vitro--Analysis of RNA binding proteins using in Vitro genetics--Interactions of proteins with specific sequences in RNA--A rapid genetic method for the study of RNA binding proteins--Footprinting RNA-protein complexes with hydroxyl radicals--Analysis of ribosome loading onto mRNA species: implications for translational control--Analysis of Picornavirus internal ribosome entry site function in Vivo--RNA traffic and localization reported by fluorescent molecular cytochemistry in living cells-- Detection of mRNA in Situ: techniques for studying gene expression in Drosophila melanogaster tissues--Differential display protocol that preferentially identifies mRNAs of moderate to low abundance in a microscopic system
