Protein lipidation protocols

In Vitro Analysis of GPI Biosynthesis in Mammalian Cells / Victoria L. Stevens -- Selection of Mammalian Cell Mutants in GPI Biosynthesis / Victoria L. Stevens -- Analysis of the Cell-Surface Distribution of GPI-Anchored Proteins / Satyajit Mayor -- Imaging Fluorescence Resonance Energy Transfer as Probe of Membrane Organization and Molecular Associations of GPI-Anchored Proteins / Anne K. Kenworthy and Michael Edidin -- Purification of Caveolae-Derived Membrane Microdomains Containing Lipid-Anchored Signaling Molecules, Such as GPI-Anchored Proteins, H-Ras, Src-Family Tyrosine Linases, eNOS and G-Protein a-, b-, and g-Subunits / Michael P. Lisanti, Massimo Sargiacomo, and Philipp E. Scherer -- Analysis of Lipids in Caveolae / Jun Liu and Jan E. Schnitzer -- Analysis of the Carbohydrate Components of Glycosylphosphatidylinositol Structures Using Fluorescent Labeling / Nicole Zitzmann and Michael A. J. Ferguson -- Analysis of the Lipid Moiety of GPI Anchor in the Yeast Saccharomyces cerevisiae / Fulvio Reggiori, Elisabeth Canivenc-Gansel, and Andreas Conzelmann -- Rapid Identification of Cysteine-Linked Isoprenyl Groups By Metabolic Labeling with [3H]Farnesol and [3H]Geranylgeraniol / Douglas A. Andres, Dean C. Crick, Brian S. Finlin, and Charles J. Waechter -- Incorporation of Radiolabeled Prenyl Alcohols and Their Analogs into Mammalian Cell Proteins: A Useful Tool for Studying Protein Prenylation / Alberto Corsini, Christopher C. Farnsworth, Paul McGeady, Michael H. Gelb, and John A. Glomset -- Reconstitution of Yeast Farnesyltransferase from Individually Purified Subunits / Jun Urano and Fuyuhiko Tamanoi -- Probing the Role of H-Ras Lipidation for Signaling Functions in Xenopus laevis Oocytes / Thomas Dudler and Michael H. Gelb -- Fluorescence Measurement of Lipid-Binding Affinity and Interbilayer Transfer of Bimane-Labeled Lipidated Peptides / John R. Silvius -- Determination of the Kinetics of Intervesicle Transfer and Transbilayer Diffusion of Bimane-Labeled Lipidated Peptides / John R. Silvius -- Preparation and Assay of Myristoyl-CoA: Protein N-Myristoyltransferase / Rajala V. S. Raju and Rajendra K. Sharma -- Metabolic Labeling of Protein-Derived Lipid Thioesters in Palmitoyl-Protein Thioesterase-Deficient Cells / Sandra L. Hofmann and Jui-Yun Lu. Fatty Acid Analysis of Protein-Derived Lipid Thioesters Isolated from Palmitoyl-Protein Thioesterase-Deficient Cells / Sandra L. Hofmann and Linda A. Verkruyse.