Calcium signaling protocols

Part I. General -- 1. Fluorescent Measurement of [Ca2+]c : Basic Practical Considerations / Alec W.M. Simpson -- 2. Measurement of [Ca2+]i in Whole Cell Suspensions Using Fura-2 / Robert A. Hirst ... [et al.] -- 3. Measurement of [Ca2+]i in Cell Suspensions Using lndo-1 / Adriaan Nelemans -- Part II. Specialist Measurement Systems -- 4. Confocal Microscopy : Theory and Applications / Laura C. Mongan ... [et al.] -- 5. Measurement of Intracellular Calcium Concentration Using Confocal Microscopy / Carmen Perez-Terzic, Marisa Jaconi, and Lisa Stehno-Bittel -- 6. Single Cell and Subcellular Measurement of Intracellular Ca2+ Concentration ([Ca2+]i) / Anthony J. Morgan and Andrew P. Thomas -- 7. Measurement of [Ca2+] Using the Fluorometric Imaging Plate Reader (FLIPR) / Elaine Sullivan, Emily M. Tucker, and Ian L. Dale -- Part III. Nonelectrophysiological Measurement of Ca2+ Channel Activity -- 8. Measurement of Ca2+ Entry Using 45Ca2+ / Mercedes Villaroya ... [et al.] -- 9. Measurement of [3H]PN200-110 and [125I]w-Conotoxin MVIIA Binding / Kazuyoshi Hirota and David G. Lambert -- Part IV. Measurement of INS(1,4,5)P3 and Ca2+ Release from Intracellular Stores -- 10. Measurement of Inositol (Poly)Phosphate Formation Using [3H]Inositol Labeling Protocols in Permeabilized Cells / Philip Swigart and Shamshad Cockcroft -- 11. Measurement of Inositol(1,4,5)Trisphosphate Using a Stereospecific Radioreceptor Mass Assay / Darren Smart -- 12. Measurement of Calcium Fluxes in Permeabilized Cells Using a45Ca2+ Uptake and Release Assay / Robert A. Wilcox -- 13. Microinjection of Myo-Inositol(1,4,5)Trisphosphate and Other Calcium-Mobilizing Agents into Intact Adherent Cells / Robert A. Wilcox, Ian D. Forsythe, and Terence J. McCann -- 14. Photolysis of Caged Calcium Using a Low-Cost Flash Unit / Gommert A. van Koeveringe and Ron van Mastrigt -- 15. Measurement of Ca2+ Flux through Ins(1,4,5)P3 Receptor-Ca2+ Channels in Lipid Bilayers ("Dip-Tip" and "Schindler" Methodology) / Edwin C. Thrower -- 16. Continuous Fluorescent Monitoring of Cellular Calcium Fluxes: A Novel Perfusion System for the Investigation of Inositol (1,4,5)Triphosphate-Dependent Quantal Calcium Release Using Immobilized, Electropermealized Cells / Robert A. Wilcox and James Strupish -- 17. Measurement of Free [Ca2+] Changes in Agonist-Sensitive Internal Stores Using Compartmentalized Fluorescent Indicators / Aldebaran M. Hofer -- Part V. Specialized Measurement Techniques -- 18. Measurement of [Ca2+]i in Smooth Muscle Strips Using Front-Surface Fluorimetry / Hideo Kanaide -- 19. Measurement of Calcium and Movement in Heart Cells / Leong L. Ng and Paulene A. Quinn -- 20. Simultaneous Analysis of Intracellular pH and Ca2+ from Cell Populations / Paul Martinez-Zaguilan ... [et al.] -- 21. Measurement of Cytosolic-Free Ca2+ in Plant Tissue / Martin R. McAinsh and Irina Staxen -- Part VI. Ca2+ Sensitive Targets -- 22. Assay and Purification of Calmodulin-Dependent Protein Kinase / Rakesh Kakkar and Rajendra K. Sharma -- 23. Measurement of Ca2+-ATPase Activity (in PMCA and SERCA1) / Danuta Kosk-Kosicka.