Mycoplasma protocols

Medical significance of mycoplasmas / Paul Taylor -- The veterinary significance of mycoplasmas / Robin Nicholas -- Recovery of human mycoplasmas / Paul Taylor -- Recovery of mycoplasmas from animals / Robin Nicholas, Samantha Baker -- Recovery of mycoplasmas from birds / Janet M. Bradbury -- Cultivation of ureaplasmas / Patricia M. Furr -- Quality-control testing of mycoplasma medium / David Windsor, Helena Windsor -- Biochemical characteristics in mycoplasma identification / José B. Poveda -- Enzyme analysis : the rationale and use of enzyme assays in assigning function to gene nucleotide sequences and the procedures for the assay of three enzymatic functions conserved in mollicutes / J. Dennis Pollack -- Determination of substrate utilization rates by mycoplasmas / Roger J. Miles, Calista Agbanyim -- Serological identification of mycoplasmas by growth and metabolic inhibition tests / José B. Poveda, Robin Nicholas -- Identification of mycoplasmas by dot immunobinding on membrane filtration (MF Dot) / Francois Poumarat -- Identification of mycoplasmas by immunofluorescence / Janet M. Bradbury -- Diagnostic application of monoclonal antibody (MAb)-based sandwich ELISAs / Hywel J. Ball, David Finlay -- Immunohistochemical staining of fixed tissues / Eugenio Scanziani -- Extraction of DNA from mycoplasmas / John B. Bashiruddin -- Characterization of mycoplasmas by PCR and sequence analysis with universal 16S rDNA primers / Karl-Erik Johansson, Malin U. Heldtander, Bertil Pettersson -- PCR and RFLP methods for the specific detection and identification of Mycoplasma mycoides subsp. mycoides SC / John B. Bashiruddin -- Characterization of mycoplasmas by RAPD fingerprinting / Georges A. Rawadi -- Classification of isolates by DNA-DNA hybridization / Konrad Sachse, Helmut Hotzel -- Insertion sequence analysis / Joachim Frey -- Detection of mycoplasmas in cell cultures by cultural methods / Gerald K. Masover, Frances A. Becker -- Detection of mycoplasmas in cell cultures by fluorescence methods / Gerald K. Masover, Frances A. Becker -- Transformation of mycoplasmas / Chris F. Minion, Paul A. Kapke -- Transposon mutagenesis / LeRoy L. Voelker, Kevin Dybvig -- Demonstration of extrachromosomal elements / LeRoy L. Voelker, Kevin Dybvig -- Expression of foreign genes in Acholeplasma laidlawii / Tanja K Jarhede, Åke Wieslander -- Mycoplasma gene expression in Escherichia coli / Chris F. Minion -- Polyacrylamide gel-electrophoresis separation of whole-cell proteins / Michael F. Duffy, Amir H. Noormohammadi, Nina Baseggio, Glenn F. Browning, Philip F. Markham -- Immunological and biochemical characterization of membrane proteins / Michael F. Duffy, Amir H. Noormohammadi, Nina Baseggio, Glenn F. Browning, Philip F. Markham -- Detection and analysis of mycoplasma adhesins / Konrad Sachse -- Transmission electron microscopy and immunogold staining of mollicute surface antigens / Gunna Christiansen, Svend Birkelund