Molecular embryology : methods and protocols

pt. 1. The mouse embryo -- The mouse as a developmental model / Paul T. Sharpe -- Culture of postimplantation mouse embryos / Paul Martin and David L. Cockroft -- Organ culture in the analysis of tissue interactions / Irma Thesleff and Carin Sahlberg -- Treatment of mice with retinoids in vivo and in vitro : skeletal staining / Gillian M. Morriss-Kay -- Cell grafting and labeling in postimplantation mouse embryos / Gabriel A. Quinlan, Paul A. Trainor, and Patrick P. L. Tam -- Production of transgenic rodents by the microinjection of coloned DNA into fertilized one-celled eggs / San Ling Si-Hoe and David Murphy -- Cre recombinase mediated alterations of the mouse genome using embryonic stem cells / Anna-Katerina Hadjantonakis, Melinda Pirity, and Andras Nagy -- Gene trapping in mouse embryonic stem cells / Jane Brennan and William C. Skarnes -- Production and growth of conditionally immortal cell lines from the H-2KbtsA58 transgenic mouse / Mark Noble -- Reporter genes for the study of transcriptional regulation in transgenic mouse embryos / Jonathan D. Gilthorpe and Peter W. J. Rigby -- Application of IacZ transgenic mice to cell lineage studies / Paul A. Trainor ... [et al.] -- Mouse primordial germ cells : isolation and in vitro culture / Patricia A. Labosky and Brigid L. M. Hogan -- pt. 2. The chicken embryo -- The avian embryo: an overview / Ivor Mason -- Chick embryos : incubation and isolation / Ivor Mason -- New culture / Amata Hornbruch -- Grafting Hensen's node / Claudio D. Stern -- Grafting of Somites / Claudio D. Stern -- Notochord grafts / Andrew Lumsden and Susanne Dietrich -- Transplantation of avian neural tissue / Sarah Guthrie -- Grafting of apical ridge and polarizing region / Cheryl Tickle -- Tissue recombinations in collagen gels / Marysia Placzek and Kim Dale -- Quail-chick chimeras / Marie-Aime Teillet, Catherine Ziller, and Nicole M. Le Douarin -- Using fluorescent dyes for fate mapping, lineage analysis, and axon tracing in the chick embryo / Jonathan D. W. Clarke -- pt. 3. Amphibian embryo -- An overview of Xenopus development / C. Michael Jones and Jim Smith -- Mesoderm induction assays / C. Michael Jones and Jim Smith -- Experimental embryological methods for analysis of neural induction in the amphibian / Ray Keller, Ann Poznanski, and Tamira Elul -- A method for generating transgenic frog embryos / Enrique Amaya and Kristen L. Kroll -- Axolotl/newt / Malcolm Maden -- pt. 4. Zebrafish -- The Zebrafish : An Overview of Its Early Development / Nigel Holder and Qiling Xu -- Small-Scale Marker-Based Screening For Mutations in Zebrafish Development / Peter D. Currie, Thomas F. Schilling, and Philip W. Ingham -- Transgenic Zebrafish / Trevor Jowett -- Microinjection of DNA, RNA, and Protein into the Fertilized Zebrafish Egg For Analysis of Gene Function / Nigel Holder and Qiling Xu -- Retinoids in Nonmammalian Embryos / Malcolm Maden -- Part V. Nonvertebrate Chordates -- Protochordates / Peter W. H. Holland and Hiroshi Wada -- Part VI. Retrovirus -- Gene Transfer to the Rodent Embryo by Retroviral Vectors / Grace K. Pavlath and Marla B. Luskin -- Gene Transfer in Avian Embryos Using Replication-Competent Retroviruses / Cairine Logan and Philippa Francis-West -- Part VII. Molecular Techniques -- Subtractive Hybridization and Construction of cDNA Libraries / Bruce Blumberg and Juan Carlos Izpisua Belmonte -- Differential Display of Eukaryotic mRNA / Antonio Tugores and Juan Carlos Izpisua Belmonte -- RT-PCR on Embryos Using Degenerate Oligonucleotide Primers / Anthony Graham -- Single-Cell RT-PCR cDNA Subtraction / Damian L. Weaver ... [et al.] -- In Situ Hybridization of Radioactive Riboprobes to RNA in Tissue Sections / Radma Mahmood and Ivor Mason -- In Situ Hybridization to RNA in Whole Embryos / Huma Shamim, Radma Mahmood, and Ivor Mason -- Wholemount In Situ Hybridization to Xenopus Embryos / C. Michael Jones and James C. Smith -- Wholemount In Situ Hybridization to Amphioxus Embryo / Peter W. H. Holland -- In Situ Hybridization to Sections (Nonradioactive) / Maria Rex and Paul J. Scotting -- Immunohistochemistry Using Polyester Wax / Andrew Kent -- Immunohistochemistry on Whole Embryos / Ivor Mason -- Whole Embryo Assays for Programmed Cell Death / Anthony Graham -- Gene Interference Using Antisense Oligodeoxynucleotides on Whole Chick Embryos : Optimal Ring and Roller-Bottle Culture Technique / Jonathan Cooke and Alison Isaac -- Protein Techniques : Immunoprecipitation, In Vitro Kinase Assays, and Western Blotting / David I. Jackson and Clive Dickson -- Part VIII. Microscopy and Photography -- Microscopy and Photomicrography Techniques / Richard J. T. Wingate