Isolation and Purification of Proteins
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Rajni Hatti-Kaul; Bo Mattiasson
- ناشر : Hoboken : Marcel Dekker Inc
- چاپ و سال / کشور: 2003
- شابک / ISBN : 9780824747596
Description
Preface; Contents; Contributors; Introduction; Release of Protein from Biological Host; Chromatography: The High- Resolution Technique for Protein Separation; Selectivity in Affinity Chromatography; Genetic Approaches to Facilitate Protein Purification; Solid-Liquid Separation; Membranes for Protein Isolation and Purification; Precipitation of Proteins: Nonspecific and Specific; Protein Crystallization for Large-Scale Bioseparation; Extraction for Rapid Protein Isolation; Adsorption as an Initial Step for the Capture of Proteins; Fast Chromatography of Proteins. Novel Approaches to the Chromatography of ProteinsScale-up and Commercial Production of Recombinant Proteins; Process Monitoring During Protein Purification; Protein Formulation; Regulatory Requirements, Cleaning, and Sanitization Issues in Protein Purification; Index
This publication details the isolation of proteins from biological materials; techniques for solid-liquid separation, concentration, crystallization, chromatography, scale-up, process monitoring and product formulation; and regulatory and commercial considerations in protein production
