The chemistry of oils and fats : sources, composition, properties and uses

reface xiii Abbreviations xv 1 Oils and fats: sources and constituents 1 1.1 Introduction 1 1.2 Specifications 1 1.3 Major oils and fats 2 1.3.1 Castor oil 3 1.3.2 Cocoa butter 3 1.3.3 Coconut oil 3 1.3.4 Corn oil 4 1.3.5 Cottonseed oil 5 1.3.6 Groundnut oil 5 1.3.7 Linseed (flaxseed, linola) 5 1.3.8 Olive oil 6 1.3.9 Palm oil 6 1.3.10 Palmkernel oil 7 1.3.11 Rapeseed oil (also called canola oil) 7 1.3.12 Rice bran oil 8 1.3.13 Safflower oil 9 1.3.14 Sesame oil 9 1.3.15 Soybean oil 9 1.3.16 Sunflower oil 11 1.3.17 Tall oil 11 1.3.18 Oils with modified fatty acid composition 12 1.4 Minor vegetable oils 12 1.4.1 Cocoa butter alternatives 13 1.4.2 Oils containing -linolenic acid and/or stearidonic acid 13 1.4.3 Avocado oil (Persea americana) 14 1.4.4 Chinese vegetable tallow and stillingia oil (Sapium sebiferum and Stillingia sebifera) 14 1.4.5 Crambe (Crambe abyssinica and C. hispanica) 15 1.4.6 Cuphea 15 1.4.7 Dimorphotheca 15 1.4.8 Hemp (Marijuana, Cannabis sativa) 15 1.4.9 Gold of Pleasure (Camelina sativa, also called false flax) 16 1.4.10 Hazelnut (Corylus avellana, also called filberts) 16 1.4.11 Honesty (Lunaria annua) 16 Contents 1.4.12 Lesquerella oils 16 1.4.13 Marigold (Calendula officinalis) 17 1.4.14 Meadowfoam (Limnanthes alba) 17 1.4.15 Sea buckthorn (Hippophae rhamnoides) 17 1.4.16 Tung oil (Aleurites fordii) 18 1.4.17 Walnut (Juglans regia) 18 1.4.18 Wheatgerm (Triticum aestivum) 18 1.5 Animal fats 18 1.5.1 Butter fat 18 1.5.2 Lard 20 1.5.3 Tallow 20 1.5.4 Fish oils 21 1.6 Major and minor components 23 1.6.1 Fatty acids, glycerol esters and waxes 23 1.6.2 Phospholipids 25 1.6.3 Sterols 26 1.6.4 Tocols 26 1.6.5 Fat soluble vitamins 29 1.6.6 Chlorophyll 30 1.6.7 Hydrocarbons 30 1.6.7.1 Alkanes 30 1.6.7.2 Squalene (C30H50) 31 1.6.7.3 Carotenes 31 1.6.7.4 Polycyclic aromatic hydrocarbons 32 1.7 Oils and fats in the market place 32 Bibliography and references 34 2 Extraction, refining and processing 36 2.1 Extraction 36 2.2 Refining 38 2.2.1 Degumming 39 2.2.2 Neutralisation 39 2.2.3 Bleaching 40 2.2.4 Deodorisation and physical refining 40 2.2.5 Super-refining (chromatography) 41 2.3 Processing 41 2.3.1 Blending 42 2.3.2 Fractionation 42 2.3.2.1 Winterisation 44 2.3.2.2 Hydrophilisation 44 2.3.2.3 Urea fractionation 44 2.3.3 Hydrogenation 45 2.3.4 Interesterification with chemical catalysts 47 2.3.5 Interesterification with an enzymic catalyst 48 Bibliography and references 49 3 Structure of fatty acids and lipids 50 3.1 Introduction 50 3.2 Fatty-acid nomenclature 50 vi CONTENTS 3.3 Fatty acids ± main structural features 52 3.4 Saturated acids 52 3.4.1 Short- and medium-chain acids (4:0±14:0) 53 3.4.2 Palmitic and stearic acid 53 3.4.3 Long-chain acids 54 3.5 Monoene acids 54 3.6 Methylene-interrupted polyene acids 56 3.7 Other unsaturated acids 57 3.8 Branched chain and cyclic acids 59 3.9 Oxygenated acids 61 3.9.1 Hydroxy acids 61 3.9.2 Epoxy, furanoid and oxa acids 63 3.10 Halogenated fatty acids 64 3.11 Introduction to lipid structure 64 3.12 Acylglycerols 66 3.12.1 Monoacylglycerols 66 3.12.2 Diacylglycerols 66 3.12.3 Triacylglycerols 67 3.13 Wax esters 68 3.14 Glycosyldiacylglycerols 68 3.15 Phospholipids 69 3.16 Ether lipids 72 3.17 Sphingolipids 73 Acknowledgement 75 Bibliography and references 75 4 Chemical and biological synthesis of fatty acids and lipids 76 4.1 Fatty acid synthesis 76 4.1.1 Introduction 76 4.1.2 Partial synthesis by chain extension 76 4.1.3 Synthesis via acetylenic intermediates 77 4.1.4 Synthesis by the Wittig reaction 78 4.1.5 Other synthetic procedures 79 4.1.6 Synthesis of isotopically labelled acids 80 4.2 Acylglycerol synthesis 81 4.2.1 Introduction 81 4.2.2 C3 synthons 81 4.2.3 Acylation procedures 81 4.2.4 Acyl migration 83 4.2.5 Protecting (blocking) groups 83 4.2.6 Synthesis of racemic acylglycerols 83 4.2.7 Synthesis of enantiomeric acylglycerols 86 4.2.8 Structured lipids 86 4.3 Phospholipid synthesis 87 4.4 Sphingolipid synthesis 88 4.5 Fatty-acid biosynthesis 88 4.5.1 Introduction 88 4.5.2 De novo synthesis of saturated acids 89 4.5.3 Desaturation to monoene and polyene acids and elongation in plant systems 90 CONTENTS vii 4.5.4 Desaturation and elongation in animal systems 91 4.6 Lipid biosynthesis 92 4.6.1 Formation of triacylglycerols 92 4.6.2 Phospholipids 93 4.6.3 Sphingolipids 94 4.7 Isolation procedures 94 4.7.1 Crystallisation 94 4.7.2 Distillation under reduced pressure 94 4.7.3 Chromatography including silver ion systems 95 4.7.4 Urea fractionation 95 4.7.5 Enzymic enhancement 95 4.7.6 Chemical methods 96 Bibliography and references 98 5 Analytical procedures 100 5.1 Introduction 100 5.2 Classical analytical procedures 100 5.2.1 Introduction 100 5.2.2 Extraction 101 5.2.3 Melting behaviour 102 5.2.4 Unsaturation 102 5.2.5 Acidity, saponification and unsaponifiable material 103 5.2.6 Measurement of oxidative deterioration and of oxidative stability 104 5.3 Present-day analytical techniques 106 5.3.1 Chromatography and spectroscopy 106 5.3.2 Thin-layer chromatography (TLC) and related chromatographic systems 107 5.3.3 High performance liquid chromatography (HPLC) 108 5.3.4 Gas chromatography (GC) 108 5.3.5 NMR spectroscopy 109 5.3.6 Near-infrared and Fourier transform infrared spectroscopy 110 5.3.7 Mass spectrometry 110 5.3.8 Enzymatic procedures 112 5.4 Lipid analysis 112 5.4.1 Fatty acids 113 5.4.2 Acylglycerols 116 5.4.3 Phospholipids 122 5.4.4 Minor components (sterols and tocopherols) 122 Bibliography and references 123 6 Physical properties 124 6.1 Polymorphism, crystal structure and melting point 124 6.1.1 Alkanoic and alkenoic acids 124 6.1.2 Glycerol esters 126 6.2 Spectroscopic properties 129 6.2.1 Ultraviolet spectroscopy 129 6.2.2 Infrared spectroscopy 130 6.2.3 Electron spin resonance (ESR) spectroscopy 130 viii CONTENTS 6.2.4 1H NMR spectroscopy 130 6.2.4.1 Low resolution NMR spectroscopy 131 6.2.4.2 High resolution NMR spectroscopy 131 6.2.5 13C NMR spectroscopy 133 6.2.6 Mass spectrometry 135 6.3 Other physical properties 140 6.3.1 Density 140 6.3.2 Viscosity 140 6.3.3 Refractive index 141 6.3.4 Solubility of gases in oils 141 6.3.5 Other physical properties 141 Biblography and references 143 7 Chemical properties related to unsaturated centres 146 7.1 Hydrogenation 146 7.1.1 Catalytic hydrogenation 146 7.1.2 Other chemical reductions 148 7.1.3 Biohydrogenation 149 7.2 Oxidation through reaction with oxygen 150 7.2.1 Introduction 150 7.2.2 Autoxidation 151 7.2.3 Photo-oxidation 153 7.2.4 Hydroperoxide structures 154 7.2.4.1 Methyl oleate 155 7.2.4.2 Methyl linoleate 156 7.2.4.3 Methyl linolenate 158 7.2.4.4 Cholesterol 159 7.2.5 Decomposition of hydroperoxides to short-chain products 161 7.2.6 Other secondary reaction products 163 7.2.7 Antioxidants ± introduction 164 7.2.8 Primary and secondary antioxidants 165 7.2.9 Synthetic and natural antioxidants 166 7.3 Biological oxidation 168 7.3.1 -Oxidation 168 7.3.2 -Oxidation 169 7.3.3 !-Oxidation 169 7.3.4 Lipoxygenase 169 7.3.5 Production and function of eicosanoids 171 7.4 Other oxidation reactions 171 7.4.1 Epoxidation 171 7.4.1.1 Preparation of epoxides 173 7.4.1.2 Physical and chemical properties 174 7.4.1.3 Applications 175 7.4.2 Hydroxylation 176 7.4.3 Oxidative fission 176 7.5 Halogenation 178 7.6 Stereomutation 179 7.7 Metathesis 180 7.8 Double bond migration and cyclisation 181 CONTENTS ix 7.9 Dimerisation (dimer acids, isostearic acid, estolides, Guerbet alcohols and acids) 183 7.10 Neighbouring group participation 185 7.11 Friedel-Crafts acylation and related reactions 187 Bibliography and references 188 8 Chemical properties related to the carboxyl group 189 8.1 Introduction 189 8.2 Hydrolysis 190 8.3 Esterification 191 8.3.1 Reaction between carboxylic acids and alcohols 191 8.3.2 Acidolysis 192 8.3.3 Alcoholysis 193 8.3.4 Interesterification 195 8.3.5 Structured lipids 196 8.3.6 Lactones 198 8.4 Acid chlorides, acid anhydrides and ketene dimers 199 8.5 Peroxy acids and esters 200 8.6 Long-chain alcohols 201 8.6.1 Preparation of alcohols 201 8.6.2 Ethoxylation and propoxylation of alcohols and esters 203 8.6.3 Sulfates and other alcohol esters 204 8.6.4 Alkyl polyglycosides 205 8.6.5 Guerbet alcohols and acids 205 8.7 Acetals/ketals and orthoesters 206 8.8 Nitrogen-containing compounds 207 8.9 Sulfonation and other reactions at the -position 209 8.10 Barton reaction 210 Bibliography and references 210 9 Nutritional properties 211 9.1 Dietary fat ± sources and composition 211 9.2 Digestion, absorption and transport 214 9.3 Essential fatty acids 217 9.4 Recommendations for dietary intake 222 9.5 Cholesterol and phytosterols 224 9.6 Conjugated linoleic acid (CLA) 225 9.7 Role of fats in health and disease 226 9.7.1 Obesity 227 9.7.2 Coronary heart disease (CHD) 228 9.7.3 Diabetes 231 9.7.4 Inflammatory diseases 232 9.7.5 Psychiatric disorders 233 9.7.6 Cancer 233 9.8 Functional foods 233 9.8.1 Diacylglycerols 234 9.8.2 Phytosterols 235 Bibliography and references 236 x CONTENTS 10 Edible uses of oils and fats 238 10.1 Spreads: butter and ghee 238 10.1.1 Butter 238 10.1.2 Ghee 240 10.2 Spreads: margarine and vanaspati 241 10.2.1 Margarine 241 10.2.2 Vanaspati 244 10.3 Baking fats, doughs and shortenings 245 10.4 Frying oils and fats 246 10.5 Salad oils, mayonnaise and salad cream and French dressings 247 10.6 Chocolate and confectionery fats 248 10.7 Ice cream 252 10.8 Incorporation of vegetable oils into dairy products 253 10.9 Edible coatings and spray processing 253 10.10 Emulsifying agents 254 Bibliography and references 255 11 Non-edible uses of oils and fats 257 11.1 Introduction 257 11.2 Basic oleochemicals 258 11.2.1 Acids 258 11.2.2 Esters 259 11.2.3 Alcohols 261 11.2.4 Fatty amines 261 11.3 Surfactants 261 11.3.1 Anionic surfactants 264 11.3.1.1 Production from carboxylic acids 264 11.3.1.2 Production from alcohols 264 11.3.2 Nonionic surfactants 265 11.3.3 Ethoxylation and propoxylation of alcohols and esters 265 11.3.4 Alkyl polyglycosides 266 11.3.5 Cationic surfactants: cyanides, amines and other nitrogen- containing compounds 266 11.3.6 Gemini surfactants and cleavable surfactants 267 11.4 Personal care products 269 11.5 Lubricants 269 11.6 Biodiesel 271 11.7 Glycerol 274 11.8 Dibasic acids 274 11.9 Dimers, isostearic acid, estolides, Guerbet alcohols and acids 275 11.10 Oleochemicals from castor oil 276 11.11 Surface coatings and inks 277 11.12 Epoxides, hydroxy acids, polyurethanes 279 Bibliography and references 280 Index 282