Handbook of microbiological media for the examination of food,2nd ed

Food- and Waterborne Pathogens ............................1 Food Spoilage ..........................................................6 Microbiological Production of Food .......................7 A-1 Medium...........................................................11 AAM Medium........................................................11 Abeyta-Hunt Bark Agar.........................................11 ABY Agar ..............................................................12 AC Agar.................................................................12 AC Broth................................................................12 Acetamide Agar .....................................................12 Acetamide Agar .....................................................13 Acetamide Broth....................................................13 Acetamide Medium................................................13 Acetate Agar ..........................................................13 Acetate Broth .........................................................14 Acetate Differential Agar.......................................14 Acetobacter Agar ...................................................14 Acetobacter/Gluconobacter Agar ..........................15 Acetobacter Medium..............................................15 Acetobacter xylinum Medium................................15 Acetogen Medium..................................................15 Achromobacter Choline Medium ..........................16 Achromobacter Choline Medium, Modified..........16 Achromobacter Medium........................................16 Achromobacter Medium........................................17 Acid Broth..............................................................17 Acid Egg Medium..................................................17 Acid Products Test Broth .......................................17 Acid Tomato Broth ................................................17 Acidic Tomato Medium for Leuconostoc ..............18 Acidophilic Bacillus stearothermophilus Agar .....18 Acidophilic Bacillus stearothermophilus Broth ....18 Actidione Agar.......................................................18 AE Sporulation Medium, Modified.......................18 Aeromonas Differential Agar.................................19 Aeromonas hydrophila Medium ............................19 Aeromonas Medium...............................................20 AFPA......................................................................20 Agar Medium for Differential Enumeration of Lactic Streptococci ................20 Agar Medium P......................................................21 AK Agar No. 2.......................................................21 AKI Medium..........................................................21 Alcal Mannose Medium.........................................22 Alcaligenes Agar....................................................22 Alcaligenes Medium ..............................................22 Alcaligenes N5 Medium ........................................23 Alcaligenes NA YE Medium .................................23 Alcaligenes NB YE Agar.......................................23 Alcaligenes NB YE Broth......................................23 Alcaligenes NB YE Medium .................................23 Alginate Utilization Medium.................................23 Alkaline Bacillus Medium.....................................24 Alkaline Peptone Agar...........................................24 Alkaline Peptone Salt Broth ..................................24 Alkaline Peptone Water .........................................24 Alkaline Peptone Water .........................................25 Alkaline Polypectate Agar.....................................25 Alkaline Starch Agar .............................................25 Alkaline Xylan Agar..............................................25 Alkalophile Medium..............................................25 Alkvisco Medium ..................................................25 Allantoin Agar .......................................................26 ALOA Medium......................................................26 ALOA Medium......................................................27 Amino Acid Assay Medium..................................28 Anaerobe Agar.......................................................28 Anaerobe Agar.......................................................29 Anaerobe Medium .................................................29 Anaerobic Agar......................................................29 Anaerobic Agar......................................................30 Anaerobic Broth.....................................................30 Anaerobic CNA Agar ............................................30 Anaerobic Egg Yolk Agar......................................30 Anaerobic Egg Yolk Agar......................................31 Anaerobic TVLS Medium .....................................31 Andersen’s Pork Pea Agar .....................................31 Andrade’s Broth.....................................................32 Andrade’s Carbohydrate Broth and Indicator....................................................33 Anthranilic Acid Medium, Revised.......................33 Antibiotic Medium 1..............................................34 Antibiotic Medium 1 with Tetracycline.................34 Antibiotic Medium 1 with Tetracycline, Streptomycin, and Chloramphenicol...............34 Antibiotic Medium 2..............................................34 Antibiotic Medium 3..............................................35 Antibiotic Medium 3 Plus......................................35 Antibiotic Medium 4..............................................35 Antibiotic Medium 5..............................................35 Antibiotic Medium 6..............................................35 Antibiotic Medium 7..............................................36 Antibiotic Medium 8..............................................36 Antibiotic Medium 9..............................................36 Antibiotic Medium 10............................................36 Antibiotic Medium 11............................................36 Antibiotic Medium 12............................................37 Antibiotic Medium 13............................................37 Antibiotic Sulfonamide Sensitivity Test Agar .......................................37 Antibiotic Sulfonamide Sensitivity Test Agar .......................................37 AOAC Letheen Broth............................................38 Aolpha Medium.....................................................38 Apple Juice Yeast Extract Medium .......................39 Table of Contents APT Agar ...............................................................39 APT Broth..............................................................39 Arginine Broth .......................................................40 Arginine Broth with NaCl......................................40 Arginine Glucose Slants ........................................40 Armstrong Fusarium Medium...............................40 Ascospore Agar......................................................41 ASLA Agar ............................................................41 Asparaginate Glycerol Agar ..................................41 Asparagine Broth ...................................................41 Aspergillus Differential Medium ...........................41 ATB Acid Tomato Broth........................................41 Azide Blood Agar ..................................................42 Azide Blood Agar with Crystal Violet...................42 Azide Broth............................................................42 Azide Medium .......................................................42 B12 Culture Agar, USP...........................................43 B12 Inoculum Broth, USP ......................................43 Bacillus Agar .........................................................43 Bacillus Agar, Modified.........................................43 Bacillus Agar, 1/4 Strength....................................43 Bacillus Broth, 1/4 Strength...................................44 Bacillus cereus Medium ........................................44 Bacillus cereus Selective Agar Base......................44 Bacillus coagulans Medium ..................................44 Bacillus fastidiosus Agar .......................................45 Bacillus fastidiosus Medium..................................45 Bacillus Medium....................................................45 Bacillus stearothermophilus Broth ........................46 Bacillus stearothermophilus Defined Broth ..................................................46 Bacillus stearothermophilus Sporulation Broth ............................................46 Bacillus thermoantarcticus Medium......................46 Bacillus Xylose Salts .............................................47 BAGG Broth..........................................................47 Baird-Parker Agar..................................................48 Baird-Parker Agar, Supplemented .........................48 Balamuth Medium .................................................48 Baird-Parker Medium ............................................49 Basal Synthetic Medium........................................49 Base Layer Agar with Nutrient Overlay Agar.....................................49 BC Motility Medium ............................................50 BCM Bacillus cereus Group Plating Medium ...................................50 BCM O157:H7(+) Plating Medium ......................50 BCP Azide Broth ...................................................50 BCP D Agar ...........................................................51 BCP DCLS Agar....................................................51 Beef Extract Broth .................................................51 Beef Infusion Agar.................................................51 BG Sulfa Agar .......................................................51 Bicarbonate Agar ...................................................52 Bile Esculin Agar ..................................................52 Bile Oxalate Sorbose Broth ..................................53 Bile Salts Brilliant Green Starch Agar ..................53 Bile Salts Gelatin Agar .........................................54 Biosynth Chromogenic Medium for Listeria monocytogenes .............................54 Biotin Assay Medium ...........................................54 Bismuth Sulfite Agar ............................................54 Bismuth Sulfite Agar Wilson and Blair ................55 Bismuth Sulfite Broth ...........................................55 BL Agar ................................................................55 Blood Agar ............................................................56 Blood Agar Base ...................................................56 Blood Agar Base ...................................................57 Blood Agar with 1% NaCl ....................................57 Blood Agar Base No. 2..........................................57 Bolton Broth ..........................................................57 Bouillon Medium...................................................58 Bovine Albumin Tween 80 Medium, Ellinghausen and McCullough, Modified .......58 Bovine Albumin Tween 80 Semisolid Medium, Ellinghausen and McCullough, Modified .............................58 Bovine Serum Albumin Tween 80 Agar ...............59 Bovine Serum Albumin Tween 80 Broth .............59 Bovine Serum Albumin Tween 80 Soft Agar .......60 Brain Heart Infusion Agar ....................................60 Brain Heart Infusion Agar 0.7% ...........................61 Brain Heart Infusion Broth ....................................61 Brewer Anaerobic Agar........................................ 62 Brewer Thioglycolate Medium..............................62 Brigg’s Liver Tomato Broth...................................62 Brilliant Green Agar .............................................62 Brilliant Green Agar, Modified .............................62 Brilliant Green Agar, Modified .............................63 Brilliant Green Agar with Sulfadiazine ................63 Brilliant Green Bile Agar ......................................63 Brilliant Green 2%-Bile Broth, Fluorocult ............64 Brilliant Green Bile Broth ....................................64 Brilliant Green Broth ............................................64 Brilliant Green Lactose Bile Broth .......................64 Brilliant Green Bile Broth with MUG ..................65 Brilliant Green Phenol Red Agar ..........................65 Bromcresol Purple Broth ......................................65 Bromcresol Purple Broth with NaCl .....................66 Bromcresol Purple Dextrose Broth .......................66 Bromthymol Blue Agar ........................................66 Brucella Agar ........................................................67 Brucella Agar with Vitamin K1 .............................67 Brucella Albimi Broth with 0.16% Agar...............67 Brucella Albimi Broth with 0.16% Agar and 1% Glycine ..............................................67 Brucella Anaerobic Blood Agar ...........................68 Brucella Broth ......................................................68 Brucella Broth with 0.16% Agar ..........................68 Brucella Medium Base .........................................68 Brucella Medium, Selective .................................68 Brucella Selective Medium .................................. 69 Brucella Semisolid Medium with Cysteine ..................................................69 Brucella Semisolid Medium with Glycine ...................................................69 Brucella Semisolid Medium with NaCl ................70 Brucella Semisolid Medium with Nitrate .............70 Buffered Enrichment Broth ...................................70 Buffered Peptone Water ........................................71 CAL Broth ............................................................71 Campylobacter Agar, Blaser’s.............................. 71 Campylobacter Agar, Skirrow’s ...........................71 Campylobacter Blood-Free Agar Base Modified .........................................................72 Campylobacter Blood-Free Selective Agar ..........72 Campylobacter Charcoal Differential Agar ..........72 Campylobacter Enrichment Broth ........................73 Campylobacter Enrichment Broth ........................74 Campylobacter Enrichment Broth ........................75 Campylobacter Enrichment Broth ........................76 Campylobacter fecalis Medium ............................76 Campylobacter fetus Medium ...............................77 Campylobacter fetus Selective Medium ...............77 Campylobacter Isolation Agar A ..........................78 Campylobacter Isolation Agar B ..........................78 Campylobacter Medium .......................................78 Campylobacter Selective Medium, Blaser-Wang ....................................79 Campylobacter Selective Medium, Butzler’s ..........................................80 Campylobacter Selective Medium, Karmali’s .........................................80 Campylobacter Selective Medium, Preston’s .......81 Carbohydrate Fermentation Broth ........................81 Casamino Acids Yeast Extract Broth ....................82 Cary and Blair Transport Medium........................ 82 Casamino Acids Yeast Extract Broth ....................82 Casamino Acids Yeast Extract Lincomycin Medium ..........................82 Casamino Acids Yeast Extract Salts Broth, Gorbach ..........................83 CCY Modified Medium ........................................83 Cefsulodin Irgasan Novobiocin Agar ...................83 Cell Growth Medium ............................................84 Cellobiose Polymyxin Colistin Agar ....................85 Cellobiose Polymyxin B Colistin Agar, Modified ..................................85 Cellulase Solution .................................................86 Cereal Agar ...........................................................86 Cetrimide Agar, Non-USP ....................................86 Cetrimide Agar, USP ............................................86 Chapman Stone Agar ............................................87 Cheese Agar ..........................................................87 Cherry Agar, CBS Formula ..................................87 China Blue Lactose Agar ......................................87 CHO Medium Base ...............................................87 Cholera Medium TCBS ........................................ 88 Chopped Liver Broth ............................................ 88 Chopped Meat Agar ..............................................88 Chopped Meat Broth .............................................88 Chopped Meat Carbohydrate Medium .................89 Christensen Agar ..................................................89 Christensen Citrate Agar .......................................89 Christensen Citrate Agar, Modified.......................90 Christensen Citrate Sulfide Medium ....................90 Christensen’s Urea Agar .......................................90 Christensen’s Urea Agar with NaCl .....................90 CHROMagar E. coli .............................................91 CHROMagar ECC ................................................91 CHROMagar Listeria ...........................................91 CHROMagar O157................................................91 CHROMagar Salmonella ......................................91 CHROMagar Staph. aureus ..................................92 CHROMagar Vibrio ..............................................92 Chromogenic Bacillus cereus Agar ......................92 Chromogenic E. coli/Coliform Medium ...............92 Chromogenic Enterobacter sakazakii Agar, DFI Formulation ...................................93 Chromogenic Listeria Agar ..................................93 Chromogenic Salmonella Esterase Agar ..............93 Chromogenic Substrate Broth ...............................93 CIN Agar ..............................................................94 Clostridia Medium ................................................94 Clostridium acidurici Medium .............................94 Clostridium aerotolerans Medium ....................... 95 Clostridium Alginate Medium ..............................95 Clostridium aminobutyricum Medium .................95 Clostridium aminobutyricum Medium .................96 Clostridium botulinum Isolation Agar .................. 96 Clostridium CK Medium...................................... 97 Clostridium difficile Agar .....................................97 Clostridium perfringens Agar, OPSP ...................98 Clostridium Selective Agar ...................................98 Coagulase Agar Base............................................ 98 Coagulase Mannitol Agar .....................................99 Coletsos Medium ..................................................99 Coletsos Selective Medium ..................................99 Coli ID .................................................................100 Coliform Agar, Chromocult.................................100 Coliform Agar ES, Chromocult...........................100 Coliform Medium................................................101 Coliform Medium, Modified ...............................101 Coliform Medium, Modified ...............................102 Colonization Medium..........................................102 Congo Red Agar ..................................................102 Congo Red Agar ..................................................103 Congo Red BHI Agarose Medium ......................104 Congo Red Magnesium Oxalate Agar.................104 Conradi Drigalski Agar........................................105 Cooke Rose Bengal Agar.....................................105 Cooked Meat Medium .........................................105 Cooked Meat Medium, Modified ........................106 Cornmeal Agar.....................................................106 Cornmeal Agar.....................................................106 Cornmeal Agar with Dextrose .............................107 Cornmeal and V8 Juice Agar...............................107 Cornmeal Agar, Quarter-Strength........................107 Creatinine/NMH Medium....................................107 CreDm1 Medium .................................................108 Crossley Milk Medium........................................109 Crystal Violet Agar ..............................................109 Crystal Violet Pectate Medium ............................109 CS Vitamin B12 Agar ..........................................110 CYA Agar with Arginine and p-Aminobenzoic Acid.............................110 Czapek Dox Agar, Modified................................110 Czapek Dox Broth ...............................................110 Czapek Dox Liquid Medium, Modified ..............111 Czapek Solution Agar..........................................111 Czapek Solution Agar with Sucrose ....................111 Czapek Yeast Autolysate Agar ............................111 Czapek Yeast Extract Agar ..................................111 Czapek Yeast Extract Agar ..................................112 DCLS Agar ..........................................................112 Decarboxylase Basal Medium .............................112 Decarboxylase Basal Medium with NaCl............113 Decarboxylase Base, Møller ................................113 Decarboxylase Medium Base, Falkow ................113 Decarboxylase Medium, Ornithine Modified......113 Demi-Fraser Broth ...............................................114 Deoxycholate Agar ..............................................114 Deoxycholate Citrate Agar ..................................114 Deoxycholate Citrate Agar ..................................115 Deoxycholate Citrate Agar, Hynes ......................115 Deoxycholate Lactose Agar.................................115 Dextrose Agar ......................................................116 Dextrose Agar ......................................................116 Dextrose Tryptone Agar ......................................116 Dextrose Tryptone Broth .....................................116 Dextrose Tryptone Broth .....................................117 Dichloran 18% Glycerol Agar .............................117 Dichloran-Glycerol Agar .....................................117 Dichloran Rose Bengal Chloramphenicol Agar ..................................117 Differential Broth for Lactic Streptococci ...........118 DNase Agar..........................................................118 DNase Test Agar ..................................................118 DNase Test Agar with Methyl Green...................118 DNase Test Agar with Toluidine Blue .................119 Dorset Egg Medium.............................................119 Double-Strength Crude Lactobacillus Medium...................................119 Double-Strength Crude Medium for Lactobacillus............................................119 Doyle and Roman Enrichment Medium..............120 DRBC Agar..........................................................120 Duncan-Strong Sporulation Medium, Modified.....................121 E Agar ..................................................................121 Eagle’s Minimal Essential Medium with Earle’s Salts and Nonessential Amino Acids .....................121 EB Motility Medium............................................122 EC Broth ..............................................................122 EC Broth with MUG............................................122 EC Medium, Modified with Novobiocin.............122 ECD Agar, Fluorocult..........................................123 EE Broth ..............................................................123 EE Broth, Mossel.................................................123 Egg Yolk Agar .....................................................124 Egg Yolk Agar, Modified.....................................124 Egg Yolk Emulsion..............................................124 Egg Yolk Emulsion, 50%.....................................125 Egg-Yolk Tellurite Emulsion 20%.......................125 Eijkman Lactose Medium....................................125 Elliker Agar .........................................................125 Elliker Agar .........................................................126 Elliker Broth ........................................................126 Elliker Lactose Broth...........................................126 EMB Agar............................................................126 EMB Agar Base...................................................127 EMB Agar, Modified...........................................127 Endo Agar............................................................127 Endo Agar............................................................128 Endo Agar, LES...................................................128 Endo Broth...........................................................128 Enriched Nutrient Agar........................................129 Enriched Nutrient Broth ......................................129 Enrichment Broth for Aeromonas hydrophila.............................129 Enrichment Broth, pH 7.3....................................129 Enrichment Broth, pH 7.3....................................130 Enrichment Broth, pH 7.3 with Pyruvate ............130 Enrichment Broth, pH 7.3 with Pyruvate ............131 Entamoeba dispar Axenic Culture Medium........131 Entamoeba Medium.............................................132 Enteric Fermentation Base...................................132 Enterobacter Medium..........................................133 Enterococci Broth, Chromocult ...........................133 Enterococci Confirmatory Agar ..........................133 Enterococci Confirmatory Broth .........................133 Enterococci Presumptive Broth ...........................134 Enterococcosel Agar............................................134 Enterococcosel Broth...........................................134 Enterococcus Agar...............................................134 Esculin Agar ........................................................135 Esculin Agar, Modified CDC ..............................135 Esculin Broth .......................................................135 Esculin Iron Agar.................................................135 Esculin Mannitol Agar.........................................135 ETGPA.................................................................136 Ethyl Violet Azide Broth .....................................136 Ethyl Violet Azide Broth .....................................137 Fay and Barry Medium........................................137 FC Agar ...............................................................137 FC Agar................................................................138 FC Broth ..............................................................138 Fecal Coliform Agar, Modified ...........................138 Fecal Coliform Agar, Modified ...........................139 Fermentation Basal Medium................................139 Fermentation Broth ..............................................139 Fermentation Medium..........................................140 FGTC Agar...........................................................140 Fish Peptone Agar................................................140 Fish Peptone Broth...............................................140 Flavobacterium Medium .....................................141 Flavobacterium Medium .....................................141 Fluid Sabouraud Medium ....................................141 Fluid Thioglycolate Agar.....................................141 Fluid Thioglycolate Agar with Calcium Carbonate................................142 Fletcher Medium..................................................142 Fletcher Medium with Fluorouracil.....................142 Fletcher’s Semisolid Medium..............................142 FLN Medium .......................................................142 Flo Agar ...............................................................143 Fluid Thioglycolate Agar with Calcium Carbonate................................143 Fluid Thioglycolate Medium ...............................143 Fluid Thioglycolate Medium with Beef Extract...........................................143 Fluid Thioglycolate Medium with K Agar ..........144 Fluid Thioglycolate Medium with Rabbit Serum.........................................144 Fluid Thioglycolate Medium without Glucose or Eh Indicator ....................144 Fluorescent Pectolytic Agar.................................144 Fluorocult E. coli O157:H7 Agar ........................145 FN Medium..........................................................145 Folic Acid Agar ...................................................145 Folic Acid Assay Medium...................................146 FPA Medium........................................................146 Fraser Broth .........................................................146 Fraser Secondary Enrichment Broth....................147 Freezing Medium.................................................147 G Medium............................................................147 GBNA Medium....................................................148 Gelatin Agar.........................................................148 Gelatin Infusion Broth .........................................148 Gelatin Medium ...................................................148 Gelatin Phosphate Salt Agar ................................149 Gelatin Phosphate Salt Broth...............................149 Gelatin Salt Agar .................................................149 Gelatinase Test Medium ......................................149 Gentamicin Sulfate Solution................................150 Giolitti-Cantoni Broth..........................................150 Gluconate Peptone Broth .....................................150 Glucose Agar with 25% Glucose.........................150 Glucose Phosphate Broth.....................................150 Glucose Salt Teepol Broth ...................................151 Glucose Salts Medium.........................................151 Glucose Yeast Extract Agar .................................151 Glucose Yeast Extract Peptone Agar with 2% Glucose ....................151 Glucose Yeast Extract Peptone Medium .............151 Glucose Yeast Medium with Calcium Carbonate................................152 Glutamate Medium..............................................152 Glutamine Medium..............................................152 Glutarate Medium................................................152 GN Broth, Hajna..................................................153 Green Yeast and Mold Broth ...............................153 GSP Agar .............................................................153 Gum Tragacanth Gum Arabic Medium ...............154 GV Medium.........................................................154 H Agar .................................................................155 H Broth ................................................................155 H Medium............................................................155 H Top Agar ..........................................................155 Halophile Medium ...............................................155 Halophilic Agar ...................................................156 Halophilic Broth ..................................................156 Halophilic Nutrient Agar .....................................156 Halophilic Synthetic Medium..............................156 Ham’s F-10 Medium............................................156 Heart Infusion Agar ......................................