Protein purification protocols

General strategies / Shawn Doonan -- Preparation of extracts from animal tissues / Shawn Doonan -- Protein extraction from plant tissues / Peter R. Shewry, Roger J. Fido -- Extraction of recombinant protein from bacteria / D. Margaret Worrall -- Protein extraction from fungi / Paul Bridge -- Subcellular fractionation of animal tissues / Norma M. Ryan -- Subcellular fractionation of plant tissues : isolation of chloroplasts and mitochondria from leaves / Alyson K. Tobin -- The extraction of enzymes from plant tissues rich in phenolic compounds / William S. Pierpoint -- Avoidance of proteolysis in extracts / Robert J. Beynon, Simon Oliver -- Concentration of extracts / Shawn Doonan -- Making and changing buffers / Shawn Doonan -- Purification and concentration by ultrafiltration / Paul Schratter -- Bulk purification by fractional precipitation / Shawn Doonan -- Ion-exchange chromatography / David Sheehan, Richard FitzGerald -- Hydrophobic interaction chromatography / Paul O'Farrell -- Affinity chromatography / Paul Cutler -- Dye-ligand affinity chromatography / D. Margaret Worrall -- Lectin affinity chromatography / Iris West, Owen Goldring -- Immunoaffinity chromatography / George W. Jack, David J. Beer -- Immobilized metal ion affinity chromatography / Tai-Tung Yip, T. William Hutchens -- Chromatography on hydroxyapatite / Shawn Doonan -- Affinity precipitation methods / Jane A. Irwin, Keith F. Tipton -- Isoelectric focusing / Reiner Westermeier -- Chromatofocusing / Timothy J. Mantle, Patricia Noone -- Size-exclusion chromatography / Paul Cutler -- Fast protein liquid chromatography (FPLC) methods / David Sheehan -- Reversed-phase chromatography of proteins / Bill Neville -- Extraction of membrane proteins / Kay Ohlendieck -- Removal of detergent from protein fractions / Kay Ohlendieck -- Purification of membrane proteins / Kay Ohlendieck -- Lyophilization of proteins / Ciarán Ó. Fágáin -- Storage of pure proteins / Ciarán Ó. Fágáin -- Electroelution of proteins from polyacrylamide gels / Michael J. Dunn -- Electroblotting of proteins from polyacrylamide gels / Michael J. Dunn -- High-performance electrophoresis chromatography / Serge Desnoyers, Sylvie Bourassa, Guy G. Poirier -- Practical column chromatography / Shawn Doonan