Mycobacteria protocols

Mycobacteria : bugs and bugbears / Tanya Parish, Neil G. Stoker -- Mycobacteria : general culture methodology and safety considerations / Bryan W. Allen -- Isolation of genomic DNA from mycobacteria / John T. Belisle, Michael G. Sonnenberg -- Nucleic-acid extraction : plasmid/cosmid DNA / Bonnie B. Plikaytis, C. Harold King -- Pulsed field gel electrophoresis for mycobacteria / Wolfgang J. Philipp, Stephen Gordon, Amalio Telenti, Stewart T. Cole -- Extraction of RNA from mycobacteria / Eshwar Mahenthiralingam -- Preparation of cell-free extracts from mycobacteria / Tanya Parish, Paul R. Wheeler -- Preparation of cell-wall fractions from mycobacteria / Gurdyal S. Besra -- Using MycDB on the World-Wide Web / Staffan Bergh, Stewart T. Cole -- Conjugating DNA into mycobacteria / Julie-Ann Gavigan, Carlos Martín -- Electroporation of mycobacteria / Tanya Parish, Neil G. Stoker -- Mycobacteriophages / Gary J. Sarkis, Graham F. Hatfull -- Chemical mutagenesis of mycobacteria / Lucy A. Brooks -- Tn611 transposon mutagenesis in Mycobacterium smegmatis using a temperature-sensitive delivery system / Esther Pérez, Julie-Ann Gavigan, Isabel Otal, Christophe Guilhot, Vladimir Pelicic, Brigitte Giquel, Carlos Martin -- Homologous recombination in Mycobacterium smegmatis : screening methods for detection of gene replacement / Robert N. Husson -- Gene replacement in Mycobacterium smegmatis using a dominant negative selectable marker / Peter Sander, Erik C. Böttger -- Gene replacement in Mycobacterium intracellulare / Britt-Inger Marklund, Richard W. Stokes -- Gene replacement in Mycobacterium bovis BCG / Elizabeth Norman -- The application of bacterial luciferase as a reporter gene in mycobacteria / Stephen Gordon, Gavin Chung, Peter Andrew -- Green fluorescent protein : a tool for gene expression and cell biology in mycobacteria / Laura E. Via, Subramanian Dhandayuthapani, Dusanka Deretic, V. Deretic -- Expression of genes in mycobacteria / Peadar Ó Gaora -- Secretion of mammalian proteins from mycobacteria / Peter J. Murray, Richard A. Young -- Intracellular gene expression : analysis of RNA from mycobacteria in macrophages using RT-PCR / Philip D. Butcher, Joseph A. Mangan, Irene M. Monahan -- Analysis of mycobacterial differential gene expression by RAP-PCR / Joseph A. Mangan, Philip D. Butcher -- Mycobacterial speciation / Francis A. Drobniewski, Anne H. Uttley -- Species identification of mycobacteria using rDNA sequencing / Philip Kirschner, Erik C. Böttger -- Detection of Mycobacterium tuberculosis by a calorimetric polymerase chain reaction / Stuart M. Wilson -- Rapid detection and simultaneous strain differentiation of Mycobacterium tuberculosis complex bacteria by spoligotyping / Henri O. Molhuizen, Annelies E. Bunschoten, Leo M. Schouls, Jan D. Embden -- Computer analysis of IS6110 RFLP patterns of Mycobacterium tuberculosis / Herre F. Heersma, Kristin Kremer, Jan D. Embden -- Detection of mutations in mycobacteria by PCR-SSCP (single-strand conformation polymorphism) / Amalio Telenti, Nadine Honoré, Stewart T. Cole -- The use of luciferase-reporter phage for antibiotic-susceptibility testing of mycobacteria / Paul F. Riska, William R. Jacobs