Plant cell culture protocols

TABLE OF CONTENTS -- An introduction to plant-cell culture : pointers to success -- Callus initiation, maintenance and shoot induction in rice -- Callus initiation, maintenance and shoot induction in potato : monitoring of spontaneous genetic viability in vitro and in vivo -- Somatic embryogenesis in barley suspension cultures -- Direct, cyclic somatic embryogenesis of cassava for mass production purposes -- Immature inflorescence culture of cereals : a highly responsive system for regeneration and transformation -- Cryopreservation of rice tissue cultures -- Noncryogenic, long-term germplasm storage -- Micropropagation of strawberry via axillary shoot proliferation -- Meristem-tip culture for propagation and virus elimination -- Clonal propagation of orchids -- In vitro propagation of succulent plants -- Micropropagation of flower bulbs : lily and narcissus -- Clonal propagation of woody species -- Spore-derived axenic cultures of ferns as a method of propagation. TABLE OF CONTENTS CONT'D. -- Protoplast isolation, culture and plant regeneration from passiflora -- Isolation, culture, and plant regeneration of suspension-derived protoplasts of lolium -- Protoplast fusion for symmetric somatic hybrid production in brassicaceae -- Production of cybrids in rapeseed (brassica napus) -- Microprotoplast-mediatedd chromosome transfer (MMCT) for the dircet production of monosomic addition lines -- Guard cell protoplasts : isolation, culture, and regeneration of plants -- In virto fertilization with isolated single gametes -- Protocols for anther and microspore culture of barley -- Microspore embryogenesis and in vitro pollen maturation in tobacco -- Embryo rescue following wide crosses -- Mutagenesis and the selection of resistant mutants -- The generation of plastid mutants in vitro -- Agrobacterium-mediated transformation of petunia leaf disks -- Transformation of rice via PEG-mediated DNA uptake into protoplasts -- Transformation of wheat via particle bombardment -- Plant transformation via protoplast electroporation -- Transformation of maize via tissue electroporation -- Transformation of maize using silicon carbide whiskers -- Directing anthraquinone acculumation via manipulation of morinda suspension cultures -- Alkaloid accumulation in catharanthus roseus suspension cultures -- Betalains : their accumulation and release in vitro