Bacterial toxins : methods and protocols
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Otto Holst
- ناشر : Totowa, N.J. : Humana Press
- چاپ و سال / کشور: 2000
- شابک / ISBN : 9780896036048
Description
Bacterial protein toxins: an overview -- Purification of clostridium botulinum type A neurotoxin -- Shiga toxins -- Isolation and detection of microcystins and nodularins, cyanobacterial peptide hepatotoxins -- Genetic construction, expression, and characterization of diphtheria toxin-based growth factor fusion proteins -- Structure-function analysis of cysteine-engineered entomopathogenic toxins -- Use of fourier-transformed infrared spectroscopy for secondary structrue determination of staphyloccoccal pore-forming toxins -- The use of fluorescence resonance energy transfer to detect conformational changes in protein toxins -- Site-directed spin labeling of proteins: applications to diphtheria toxin -- Characterization of molecular properties of pore-forming toxins with planar lipid bilayers --Determination of affinity and kinetic rate constants using surface plasmon resonance -- ADP-ribosylation of [alpha]-Gi proteins by pertusis toxin: positional dissection of acceptor sites using membrane anchored synthetic peptides -- Phage libraries for generation of anti-botulinum scFv antibiodies -- T-cell cytotoxicity assays for studying the functional itneraction between the superantigen staphylococcal enterotoxin A and T-cell receptors -- In vitro physiological studies on clostridial neurotoxins: biological modesl and procedures for extracellular and intracellular application of toxins -- The biology of endotoxin -- Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of lipopolysaccharides -- Applications of combined capillary electrophoresis-electrospray mass spectrometry in the characterization of short-chain lipopolysaccharides: haemophilus influenzae -- Deacylation of lipopolysaccharides and isolation of oligosaccharide phosphates -- Electrophysiological measurement on reconstituted outer membranes
