Stress response : methods and protocols

Identifying and counting protein modifications triggered by nitrosative stress / Prabodh K. Sehajpal, Harry M. Lander -- Determination of carbonyl groups in oxidized proteins / Rodney L. Levine, Nancy Wehr, Joy A. Williams, Earl R. Stadtman, Emily Shacter -- Quantitation of 4-hydroxynonenal protein adducts / Koji Uchida, Earl R. Stadtman -- Detection of oxidative stress in lymphocytes using dichlorodihydrofluorescein diacetate / Cecile M. Krejsa, Gary L. Schieven -- The measurement of protein degradation in response to oxidative stress / Thomas Reinheckel, Tilman Grune, Kelvin J. Davies -- Analysis of the role of the AMP-activated protein kinase in the response to cellular stress / D. Grahame Hardie, Ian P. Salt, Stephen P. Davies -- Detection and activation of stress-responsive tyrosine kinases / Gary L. Schieven -- Detection of DNA-dependent protein kinase in extracts from human and rodent cells / Yamini Achari, Susan P. Lees-Miller -- Expression and assay of recombinant ATM / Yael Ziv, Sharon Banin, Dae-Sik Lim, Christine E. Canman, Michael B. Kastan, Yosef Shiloh -- Detection and purification of a multiprotein kinase complex from mammalian cells : IKK Signalsome / Frank Mercurio, David B. Young, Anthony M. Manning -- Methods to assay stress-activated protein kinases / Ana Cuenda -- Monitoring the activation of stress-activated protein kinases using GAL4 fusion transactivators / Chao-Feng Zheng, Li Xu -- Use of kinase inhibitors to dissect signaling pathways / Ana Cuenda, Dario R. Alessi -- The development and use of phospho-specific antibodies to study protein phosphorylation / Jeremy P. Blaydes, Borek Vojtesek, Graham B. Bloomberg, Ted R. Hupp -- Peptide assay of protein kinases and use of variant peptides to determine recognition motifs / D. Grahame Hardie -- Assaying NF-KB and AP-1 DNA-binding and transcriptional activity / Judith M. Mueller, Heike L. Pahl -- Analysis of the mammalian heat-shock response : inducible gene expression and heat-shock factor activity / Anu Mathew, Yanhong Shi, Caroline Jolly, Richard I. Morimoto -- Approaches to define the involvement of reactive oxygen species and iron in ultraviolet-A inducible gene expression / Charareh Pourzand, Olivier Reelfs, Rex M. Tyrrell -- The human immunodeficiency virus LTR-promoter region as a reporter of stress-induced gene expression / Michael W. Bate, Sushma R. Jassal, David W. Brighty -- SAGE : the serial analysis of gene expression / Jill Powell -- Analysis of differential gene expression using the SABRE enrichment protocol / Daniel J. Lavery, Philippe Fonjallaz, Fabienne Fleury-Olela, Ueli Schibler -- UVB-regulated gene expression in human keratinocytes: analysis by differential display / Harry Frank Abts, Thomas Welss, Kai Breuhahn, Thomas Ruzicka -- Heme oxygenase activity current methods and applications / Stefan W. Ryter, Egil Kvam, Rex M. Tyrrell -- Analysis of molecular chaperone activities using in vitro and in vivo approaches / Brian C. Freeman, Annamieke Michels, Jaewhan Song, Harm H. Kampinga, Richard I. Morimoto -- Analysis of chaperone properties of small Hsp's / Monika Ehrnsperger, Matthias Gaestel, Johannes Buchner -- Analysis of small Hsp phosphorylation / Rainer Benndorf, Katrin Engel, Matthias Gaestel -- Analysis of multisite phosphorylation of the p53 tumor-suppressor protein bytryptic phosphopeptide mappin / David W. Meek, Diane M. Milne -- Development of physiological models to study stress protein responses / Ted R. Hupp