Extracellular matrix protocols

Semipermeabilized cells to study procollagen assembly / Richard R. Wilson, Neil J. Bulleid -- Quantitative determination of collagen crosslinks / Trevor J. Sims, Nicholas C. Avery, Allen J. Bailey -- Analysis of laminin structure and function with recombinant glycoprotein expressed in insect cells / Todd L. Mathus, Peter D. Yurchenco -- Recombinant collagen trimers from insect cells and yeast / Johanna Myllyharju -- Eukaryotic expression and purification of recombinant extracellular matrix proteins carrying the Strep II tag / Neil Smyth ... [et al.] -- Preparation of isotopically labeled recombinant fragments of fibronectin for functional and structural study by heteronuclear nuclear magnetic resonance spectroscopy / Jeremy R. Bright ... [et al.] -- Reconstitution of functional integrin into phospholipid vesicles and planar lipid bilayers / Eva-Maria Erb, Jürgen Engel -- Confocal-FRAP analysis of ECM molecular interactions / Timothy Hardingham, Philip Gribbon -- Electron cryomicroscopy of fibrillar collagens / Roger S. Meadows ... [et al.] -- Atomic force microscopy measurements of intermolecular binding strength / Gradimir N. Misevic -- ECM macromolecules : rotary shadowing and scanning transmission electron microscopy / Michael J. Sherratt ... [et al.] -- Screening for mutations in cartilage ECM genes / Michael D. Briggs -- Tissue-specific KO of ECM proteins / Emilio Hirsch, Mara Brancaccio, Fiorella Altruda -- Homologous gene targeting to study ECM assembly / Francesco Ramirez, Friedrich Laub, Hideaki Sumiyoshi -- Enhancer analysis of the [alpha]1(II) and [alpha]2(XI) collagen genes in transfected chondrocytes and transgenic mice / Noriyuki Tsumaki ... [et al.] -- Retroviral delivery of ECM genes to cells / Kyonggeun Yoon, Vitali Alexeev -- Using self-assembled monolayers to pattern ECM proteins and cells on substrates / Christopher S. Chen ... [et al.] -- Methods for preparing extracellular matrix and quantifying insulin-like growth factor-binding protein binding to the ECM / Bo Zheng, David R. Clemmons -- Measuring interactions between ECM and TGF[beta]-like proteins / Sarah L. Dallas -- Using organotypic tissue slices as substrata for the culture of dissociated cells / Daniel E. Emerling, Arthur D. Lander -- Neuroepithelial differentiation induced by ECM molecules / José María Frade, Alfredo Rodriguez Tébar -- Migration assays for oligodendrocyte precursor cells / Emma E. Frost, Richard Milner, Charles ffrench-Constant -- Cell adhesion assays / Martin J. Humphries -- Tissue engineering and cell-populated collagen matrices / Paul D. Kemp -- Solid phase assays for studying ECM protein-protein interactions / A. Paul Mould -- Tissue engineering of cartilage / Ronda E. Schreiber, Anthony Ratcliffe -- Tissue recombinants to study extracellular matrix targeting to basement membranes / Patricia Simon-Assmann, Michèle Kedinger -- Fluorescence assays to study cell adhesion and migration in vitro / Paola Spessotto, Emiliana Giacomello, Roberto Perris -- Analyzing cell-ECM interactions in adult mammary gland by transplantation of embryonic mammary tissue from knockout mice / Teresa C.M. Klinowska, Charles H. Streuli