PCR protocols

A short history of the polymerase chain reaction / J.M. Bartlett and D. Stirling -- Pcr patent issues / P. Carroll and D. Casimir -- Equipping and establishing a pcr laboratory / S. McDonagh -- Quality control in pcr / D. Stirling -- Extraction of nucleic acid templates / J.M. Bartlett -- Extraction of DNA from whole blood / J.M. Bartlett and A. White -- DNA extraction from tissue / H. Pearson and D. Stirling -- Extraction of DNA from microdissected archival tissues / J.J. Going -- Rna extraction from blood / H. Pearson -- Rna extraction from frozen tissue / J.M. Bartlett -- Rna extraction from tissue sections / H. Pearson -- Dual DNA/rna extraction / D. Stirling and J.M. Bartlett -- DNA extraction from fungi, yeast, and bacteria / D. Stirling -- Isolation of rna viruses from biological materials / S. McDonagh -- Extraction of ancient DNA / W.M. Schmerer -- DNA extraction from plasma and serum / D. Stirling -- Technical notes for the detection of nucleic acids / J.M. Bartlett -- Technical notes for the recovery and purification of pcr products from acrylamide gels / D. Stirling -- Pcr primer design / D.L. Hyndman and M. Mitsuhashi -- Optimization of polymerase chain reactions / H. Grunenwald -- Subcycling pcr for long-distance amplifications of regions with high and low guanine-cystine content: Amplification of the intron 22 inversion of the fviii gene / D. Stirling -- Rapid amplification of cdna ends / X. Wang and W.S. Young, 3rd -- Randomly amplified polymorphic DNA fingerprinting: The basics / R.S. Dassanayake and L.P. Samaranayake -- Microsphere-based single nucleotide polymorphism genotyping / M.A. Iannone ... [et al.] -- Ligase chain reaction / W.H. Benjamin, Jr., K.R. Smith and K.B. Waites -- Nested rt-pcr in a single closed tube / A. Olmos ... [et al.] -- Direct pcr from serum: Application to viral genome detection / K. Abe -- Long pcr amplification of large fragments of viral genomes: A technical overview / R. Tellier ... [et al.] -- Long pcr methodology / R. Tellier ... [et al.] -- Qualitative and quantitative pcr: A technical overview / D. Stirling -- Ultrasensitive pcr detection of tumor cells in myeloma / F.W. Cremer and M. Moos -- Ultrasensitive quantitative pcr to detect rna viruses / S. McDonagh -- Quantitative pcr for camp ri alpha mrna: Use of site-directed mutation and pcr mimics / J.M. Bartlett -- Quantitation of multiple rna species / R. Kerr -- Differential display: A technical overview / J.M. Bartlett -- Au-differential display, reproducibility of a differential mrna display targeted to au motifs / O. Dominguez ... [et al.] -- Pcr fluorescence differential display / K. Khalturin, S. Kuznetsov and T.C. Bosch -- Microarray analysis using rna arbitrarily primed pcr / S. Ringquist ... [et al.] -- Oligonucleotide arrays for genotyping: Enzymatic methods for typing single nucleotide polymorphisms and short tandem repeats / S. Case-Green, C. Pritchard and E. Southern -- Serial analysis of gene expression / K.A. Oien -- Mutation and polymorphism detection: A technical overview / J. Edwards and J.M. Bartlett -- Combining multiplex and touchdown pcr for microsatellite analysis / K. Rithidech and J.J. Dunn -- Detection of microsatellite instability and loss of heterozygosity using DNA extracted from formalin-fixed paraffin-embedded tumor material by fluorescence-based multiplex microsatellite pcr / J. Edwards and J.M. Bartlett -- Reduction of shadow band synthesis during pcr amplification of repetitive sequences from modern and ancient DNA / W.M. Schmerer -- Degenerate oligonucleotide-primed pcr / M. Aubele and J. Smida -- Mutation detection using rt-pcr-rflp / H. Nakashima, M. Akahoshi and Y. Tanaka -- Multiplex amplification refractory mutation system for the detection of prothrombotic polymorphisms / D. Stirling -- Pcr-sscp analysis of polymorphism: A simple and sensitive method for detecting differences between short segments of DNA / M. Han and M.A. Robinson -- Sequencing: A technical overview / D. Stirling -- Preparation and direct automated cycle sequencing of pcr products / S.E. Daniels -- Nonradioactive pcr sequencing using digoxigenin / S. Kosel ... [et al.] -- Direct sequencing by thermal asymmetric pcr / G.R. Mazars and C. Theillet -- Analysis of nucleotide sequence variations by solid-phase minisequencing / A. Suomalainen and A.C. Syvanen -- Direct sequencing with highly degenerate and inosine-containing primers / Z. Shen ... [et al.] -- Determination of unknown genomic sequences without cloning / J.P. Quivy and P.B. Becker -- Cloning pcr products for sequencing in m13 vectors / D. Walsh -- DNA rescue by the vectorette method / M.A. McAleer, A.J. Coffey and I. Dunham -- Technical notes for sequencing difficult templates / D. Stirling -- Pcr-based detection of nucleic acids in chromosomes, cells, and tissues: Technical considerations on prins and in situ pcr and comparison with in situ hybridization / E.J. Speel, F.C. Ramaekers and A.H. Hopman -- Cycling primed in situ amplification / J.H. Bull and L. Paskins -- Direct and indirect in situ pcr / K.H. Wiedorn and T. Goldmann -- Reverse transcriptase in situ pcr: New methods in cellular interrogation / M. Gilchrist and A.D. Befus -- Primed in situ nucleic acid labeling combined with immunocytochemistry to simultaneously localize DNA and proteins in cells and chromosomes / E.J. Speel, F.C. Ramaekers and A.H. Hopman -- Cloning and mutagenesis: A technical overview / H. Pearson and D. Stirling -- Using t4 DNA polymerase to generate clonable pcr products / K. Wang -- A t-linker strategy for modification and directional cloning of pcr products / R.M. Horton, R. Raju and B.M. Conti-Fine -- Cloning gene family members using pcr with degenerate oligonucleotide primers / G.M. Preston -- Cdna libraries from a low amount of cells / P. Ravassard ... [et al.] -- Creation of chimeric junctions, deletions, and insertions by pcr / G. Pont-Kingdon -- Recombination and site-directed mutagenesis using recombination pcr / D.H. Jones and S.C. Winistorfer -- Megaprimer pcr: Application in mutagenesis and gene fusion / E. Burke and S. Barik.