DNA repair protocols : prokaryotic systems
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Pat Vaughan
- ناشر : Totowa, N.J. : Humana Press
- چاپ و سال / کشور: 2000
- شابک / ISBN : 9780896036437
Description
pt. 1. Assays to detect and quantitate DNA repair activity -- Repair of A/G and A/8-oxoG mismatches by mutY adenine DNA glycosylase -- Assays for the repair of oxidative damage by formanidopyrimidine glycosylase (Fpg) and 8-oxoguanine DNA glycosylase (OGG-1) -- Detection and quantitation of uracil DNA glycosylase activity -- Detection of DNA deoxyribophosphodiesterase activity -- O⁶-alkyguanine-DNA alkyltransferase assay -- The E. coli vsr endonuclease: assaying activity in vitro and in vivo -- Coupling of DNA helicase function to DNA strand exchange activity -- Detection and quanitation of recBCD enzymer (exonuclease V) activity -- Holiday junction branch migration and resolution: RuVA, RuvB, and RuvC from the hyperthermophile thermotoga maritima -- MutS-DNA interacitons and DNase protection analysis with surface plasmon resonance -- pt. 2. Use of DNA repair enzymes as tools in molecular biology -- Flexible genetic engineering using RecA protein -- Interspecies recombination and mismatch repair: generation of mosaic genes and genomes -- The use of immobilized mismatch binding protein in mutation/SNP detection -- Use of uracil DNA glycosylase in the detection of known DNA mutations and polymorphisms: glycosylase-mediated pholymorphism detection (GMPD-check) -- Use of uracil DNA glycosylase in scanning for unknown DNA mutations and polymorphisms: glycosylase-mediated polymorphism detection (GMPD-scan) -- The use of resolvases T4 endonuclease VII and T7 endonuclease I in mutation detection.
