Transforming growth factor-beta protocols
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Philip H Howe
- ناشر : Totowa, N.J. : Humana Press
- چاپ و سال / کشور: 2000
- شابک / ISBN : 9780896036468
Description
In vitro assays for measuring TGF-[beta] growth stimulation and inhibition -- Measurement of active TGF-[beta] generated by cultured cells -- Iodination fo active TGF-[beta], TGF-[beta]-receptor crosslinking, and immunoprecipitation of TGF-[beta]-receptor complexes -- Deterction of TGF-[beta] in body fluids and tissues -- Analysis of TGF-[beta]-mediated synthesis of extracellular matrix components -- Characterizatio of SMAD phosphorylatin and SMAD-receptor interaction -- Promoter analysis of TGF-[beta] responsive genes by transient transfection and deletional/mutational analysis -- Regulation of AP-1 activity by TGF-[beta] -- Cdk pathway: cyclin-dependent kinases and cyclin-dependent kinase inhibitors -- Activation of the mitogen-activated protein kinase pathway by transforming growth factor-[beta] -- Detection of TGF-[beta] type 11 receptor hot-spot mutations: the BAT-RII assay -- Mutation detection in the TGF-[beta] receptors and smad genes: RT-PCR and sequencing -- Analysis of TGF-[beta]-inducible apoptosis.
