SV40 protocols
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Leda Raptis
- ناشر : Totowa, N.J. : Humana Press
- چاپ و سال / کشور: 2001
- شابک / ISBN : 9780896036536
Description
Propagation of wild-type and mutant SV40 -- Propagation and assay of the JC virus -- Propagation and assay of BK virus -- Molecular methods for identification and genotyping of BK virus -- Methods for studying interactions between simian virus 40 T-antigen and the viral origin of replication -- Cell-free transcription of SV40 -- Quantitation and structural analysis of SV40 RNAs -- Generation of recombinant SV40 vectors for gene transfer -- Production of SV40 proteins in insect cells and in vitro packaging of virions and pseduovirions -- Using retroviral vectors to express SV40 tumor antigens -- Neoplastic transformation assays -- Methods to assess inhibition of adipocyte dfferentiation by SV40 large T antigen -- SV40-mediated immortalization -- Biology of p53 and SV40 large T association -- Binding of SV40 large T antigen to the retinoblastoma susceptibility gene product and related proteins -- In situ electroporation for the measurement of c-Ras activation by SVLT -- Role of SV40 small t in cell lysis, transformation, and signaling -- Cytotoxic T lymphocytes in SV40 infections -- Detection of SV40 DNA sequences in human tissue -- Guide to techniques in creating transgenic mouse models using SV40 T antigen.
