Nuclease methods and protocols

Part 1 -- Characterizing nuclease activity -- Part II -- Inhibitors and activators of nucleases -- Part III -- Relating nuclease structure and function -- Part IV -- Nucleases in the clinic -- Part V -- Assays using nucleases. Isolation of proteoglycans from cell cultures and tissues / M. Yanagishita -- Isolation of proteoglycans from tendon / K.G. Vogel and J.A. Peters -- Purification of proteoglycans from mineralized tissues / N.S. Fedarko -- Purification of perlecan from endothelial cells / J. Whitelock -- Isolation and characterization of nervous tissue proteoglycans / Y. Yamaguchi -- Analysis of proteoglycans and glycosaminoglycans from drosophila / W.D. Staatz ... [et al.] -- Cartilage and smooth muscle cell proteoglycans detected by affinity blotting using biotinylated hyaluronan / J. Melrose -- Preparation of proteoglycans for n-terminal and internal amino acid sequence analysis / P.J. Neame -- High-specific-activity 35s-labeled heparan sulfate prepared from cultured cells / N.W. Shworak -- Selective detection of sulfotransferase isoforms by the ligand affinity-conversion approach / N.W. Shworak -- Particulate and soluble glycosaminoglycan-synthesizing enzymes. Preparation assay and use / G. Sugumaran and J.E. Silbert -- Disaccharide composition of hyaluronan and chondroitin/dermatan sulfate. Analysis with fluorophore-assisted carbohydrate electrophoresis / A.H. Plaas ... [et al.] -- Integral glycan sequencing of heparan sulfate and heparin saccharides / J.E. Turnbull -- Analytical and preparative strong anion-exchange hplc of heparan sulfate and heparin saccharides / J.E. Turnbull -- Proteoglycans analyzed by composite gel electrophoresis and immunoblotting / G.R. Dodge and R. Heimer -- Quantitation of proteoglycans in biological fluids using alcian blue / M. Karlsson and S. Bjornsson -- Cellulose acetate electrophoresis of glycosaminoglycans / Y. Wegrowski and F.X. Maquart -- Disaccharide composition in glycosaminoglycans/proteoglycans analyzed by capillary zone electrophoresis / N.K. Karamanos and A. Hjerpe -- Intact and oligomeric glycosaminoglycans analyzed with capillary electrophoresis / N.K. Karamanos and A. Hjerpe -- Recombinant expression of proteoglycans in mammalian cells. Utility and advantages of the vaccinia virus/t7 bacteriophage hybrid expression system / D.J. McQuillan ... [et al.] -- Purification of recombinant human decorin and its subdomains / A.L. McBain and D.M. Mann -- Prokaryotic expression of proteoglycans / A.D. Murdoch and R.V. Iozzo -- Proteoglycan gene targeting in somatic cells / G. Ghiselli and R.V. Iozzo -- Constitutive and inducible antisense expression / M. Handler and R.V. Iozzo -- Cell-mediated transfer of proteoglycan genes / J.W. Fischer ... [et al.] -- Morphological evaluation of proteoglycans in cells and tissues / S.L. Lara, S.P. Evanko and T.N. Wight -- Expression and characterization of engineered proteoglycans / W. Luo ... [et al.] -- Intracellular localization of engineered proteoglycans / T.L. Chen ... [et al.] -- Selection of glycosaminoglycan-deficient mutants / X. Bai, B. Crawford and J.D. Esko -- Xyloside priming of glycosaminoglycan biosynthesis and inhibition of proteoglycan assembly / T.A. Fritz and J.D. Esko -- Inhibition of heparan sulfate synthesis by chlorate / H.E. Conrad -- Detection of proteoglycan core proteins with glycosaminoglycan lyases and antibodies / J.R. Couchman and P. Tapanadechopone -- Proteoglycan core proteins and catabolic fragments present in tissues and fluids / J.D. Sandy -- Degradation of heparan sulfate by nitrous acid / H.E. Conrad -- Degradation of heparan sulfate with heparin lyases / L.A. LeBrun and R.J. Linhardt -- Degradation of chondroitin sulfate and dermatan sulfate with chondroitin lyases / M.J. Hernaiz and R.J. Linhardt -- In vitro assays for hyaluronan synthase / A.P. Spicer -- Hyaluronidase activity and hyaluronidase inhibitors. Assay using a microtiter-based system / S.S. Nawy ... [et al.] -- Detecting hyaluronidase and hyaluronidase inhibitors. Hyaluronan-substrate gel and -inverse substrate gel techniques / K. Mio ... [et al.] -- Affinity coelectrophoresis of proteoglycan-protein complexes / J.D. San Antonio and A.D. Lander -- Binding constant measurements for inhibitors of growth factor binding to heparan sulfate proteoglycans / K.E. Forsten and M.A. Nugent -- Interaction of proteoglycans with receptor tyrosine kinases / D.K. Moscatello and R.V. Iozzo -- Measuring single-cell cytosolic ca2+ concentration in response to proteoglycans / S. Patel and R.V. Iozzo -- Serum amyloid a peptide interactions with glycosaminoglycans. Evaluation by affinity chromatography / J.B. Ancsin and R. Kisilevsky -- Interactions of lipoproteins with proteoglycans / K.J. Williams -- Hyaluronan and hyaluronan-binding proteins. Probes for specific detection / B.P. Toole, Q. Yu and C.B. Underhill -- Novel confocal-frap analysis of carbohydrate-protein interactions within the extracellular matrix / P. Gribbon, B.C. Heng and T.E. Hardingham -- Regulatory roles of syndecans in cell adhesion and invasion / J.K. Langford and R.D. Sanderson -- Optical biosensor techniques to analyze protein-polysaccharide interactions / D.G. Fernig -- Phage display technology to obtain antiheparan sulfate antibodies / T.H. van Kuppevelt ... [et al.] -- Tissue-specific binding by FGF and FGF receptors to endogenous heparan sulfates / A. Friedl, M. Filla and A.C. Rapraeger