Transgenesis techniques : principles and protocols

Preface ................................................................................................. v Contributors ......................................................................................... ix PART I. TOPICAL REVIEWS IN TRANSGENESIS 1 Biomedical and Agricultural Applications of Animal Transgenesis Jim McWhir ................................................................................... 3 PART II. TRANSGENESIS IN INVERTEBRATE AND LOWER VERTEBRATE SPECIES 2 Gene Transfer in Drosophila Mark J. O'Connor and William Chia ........................................ 27 PART III. TRANSGENESIS IN THE MOUSE: OOCYTE INJECTION 3 Oocyte Injection in the Mouse Gary A. J. Brown and Timothy J. Corbin ............................... 39 PART IV. ALTERNATIVE ROUTES TO THE GERMLINE 4 Adenoviral Infection Tohru Tsukui and Yutaka Toyoda ........................................... 73 5 Retroviral Infection Richard A. Bowen ...................................................................... 83 PART V. TRANSGENESIS IN THE MOUSE: THE ES CELL ROUTE 6 In Vitro Isolation of Murine Embryonic Stem Cells David Wells ................................................................................. 93 7 Production of Chimeras Derived from Murine Embryonic Stem Cells David Wells ............................................................................... 127 8 Gene Targeting Strategies David W. Melton ....................................................................... 151 9 Cre/loxP Recombination System and Gene Targeting Ralf Kühn and Raul M. Torres................................................ 175 vii Contents viii Contents PART VI. CRYOPRESERVATION OF MOUSE LINES 10 Cryopreservation of Transgenic Mouse Lines Jillian M. Shaw and Naomi Nakagata .................................... 207 11 Ovarian Tissue Transplantation and Cryopreservation: Application to Maintenance and Recovery of Transgenic and Inbred Mouse Lines Jillian M. Shaw and A. O. Trounson ...................................... 229 PART VII. TRANSGENESIS IN THE RAT 12 Transgenesis in the Rat Linda J. Mullins, Gillian Brooker, and John J. Mullins ...... 255 PART VIII. TRANSGENESIS IN DOMESTIC SPECIES 13 Generation of Transgenic Livestock by Pronuclear Injection A. John Clark............................................................................ 273 14 Transgenic Sheep from Cultured Cells Keith H. S. Campbell ............................................................... 289 PART IX. CHARACTERIZATION AND ANALYSIS OF TRANSGENIC STRAINS 15 Analysis of Transgenic Mice Stefan Selbert and Dominic Rannie ...................................... 305 Index .................................................................................................343