Neurotrophin protocols
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Robert A Rush
- ناشر : Totowa, NJ : Humana Press
- چاپ و سال / کشور: 2001
- شابک / ISBN : 9780896036994
Description
Neurotrophin quantification and localization -- RNA quantification and localization -- Construction of recombinant proteins, and transgenic cells and animals -- In vivo analysis. Localization of neurotrophin proteins within the central nervous system by immunohistochemistry / J.M. Conner -- Neurotrophin immunohistochemistry in peripheral tissues / R.A. Rush and X.F. Zhou -- Extraction and quantification of the neurotrophins / S.H. Zhang, X.F. Zhou and R.A. Rush -- Identifying novel proteins in nervous tissue using microsequencing techniques / S.E. Bartlett -- Quantification of mrna levels using northern blotting / P. Fernyhough -- Quantification of mrna levels using ribonuclease protection assay / P. Henttu -- Quantification of neurotrophin mrna by rt-pcr / E. Chie ... [et al.] -- Sensitive and nonradioactive in situ detection of neurotrophin mrnas in the nervous system / C.J. Xian and X.F. Zhou -- Expression of neurotrophic factors such as gdnf in the e. Coli system / B.R. Huang and X.M. Ma -- Construction of cells expressing neurotrophins / J. Ray and F.H. Gage -- Efficient generation of stable pheochromocytoma (pc12) cell lines using a recombinant retrovirus (lnc) / M.E. Cunningham, J.K. Kitajewski and L.A. Greene -- Construction and analysis of transgenic animals overexpressing neurotrophins / K.M. Albers and B.M. Davis -- Construction of transgenic animals overproducing neurotrophins and their receptors / C.G. Causing and F.D. Miller -- Tracing with radiolabeled neurotrophins / C.S. von Bartheld -- Toxin-induced death of neurotrophin-sensitive neurons / R.G. Wiley -- Oligonucleotides as inhibitors of protein synthesis / W.R. Epa, G.L. Barrett and P.F. Bartlett -- The iodo-gen method for labeling and the use of retrograde axonal transport to assay neurotrophins / I.A. Hendry and A.J. Reynolds -- Stereological estimation of neuronal number. The optical disector/cavalieri combination / V. Nurcombe, N.G. Wreford and J.F. Bertram