Protein kinase protocols

Protein kinase-mediated signaling networks : regulation and functional characterization / Alastair D. Reith -- Cloning protein tyrosine kinases by screening cDNA libraries with antiphosphotyrosine antibodies / Lisa D. Chong, Ira O. Daar -- Immunoprecipitation and western blotting of phosphotyrosine-containing proteins / Kathleen M. Woods Ignatoski -- Two-dimensional phosphoamino acid analysis / Peter Blume-Jensen, Tony Hunter -- Two-dimensional phosphopeptide mapping of receptor tyrosine kinases / Jeremy M. Tavar[226]e, Tarik Issad -- Identification of the sites of phosphorylation in proteins using high performance liquid chromatography and mass spectrometry / A. Grey Craig -- Phosphorylation of small signaling proteins by receptor serine/threonine kinases / Serhiy Souchelnytskyi ... [et al.] -- Assays for mitogen-activated protein kinases (MAPK) subtypes and MAPK activating protein kinase-2 (MAPKAP K-2) using a common cell lysate / Rozen Le Panse, Navita Rampersaud, Louis C. Mahadevan -- JAK-mediated phosphorylation and activation of STAT signaling proteins: analysis by phosphotyrosine blotting and EMSA / Nicola Broughton, Mark S. Burfoot -- Assays for glycogen synthase kinase-3 (GSK-3) / Darren Cross -- Cyclin-dependent kinases and cyclin-dependent kinase inhibitors: detection methods and activity measurements / Gavom Brooks -- Protein histidine kinase / Harry R. Matthews, Karina Chan -- Cloning and expression of recombinant HEXA-HIS tagged ZAP-70 using the baculovirus system / Tracey Purton, Sandra Wilkinson, Edward J. Murray -- Analysis of protein kinase subcellular localization by visualization of GTF fusion proteins / Ralph A. Zirngibi, Peter Greer -- Detection of phosphorylation-dependent interactions by far-western gel overlay / Mary Rose Burnham, Regina DeBerry, Amy H. Bouton -- Purification of tyrosine-phorylated proteins by immunoaffinity chromatography and direct cloning of their DNAs from bacterial expression libraries / Pier Paolo Di Fiore, Francesca Fazidi -- Identification of receptor tyrosine kinase (RTK) substrates by the cloning of receptor targets (CORT) strategy / Roger J. Daley -- Identification of receptor tyrosine kinase associating proteins using the yeast two-hybrid system / Sally A. Prigent -- cDNA expression cloning and characterization of phosphorylation dependent protein interactors using the yeast tribid system / Christoph Volpers ... [et al.] --tAnalysis of SH2 domain -- phosphopeptide interactions by isothermal titration calorimetry and surface plasmon resonance / George Panayotou, John Ladbury -- Cloning and characterization of RTK ligands using receptor-alkaline phosphatase fusion proteins / Hwai-Jong Cheng, John G. Flanagan -- Isolation and characterization of "orphan-RTK" ligands using an integrated biosensor approach / Martin Lackmann