Matrix metalloproteinase protocols

Part 1 -- MMPs and TIMPs -- an overview of the field -- Part II -- Expression and purification of MMPs and TIMPs -- Detection of MMPs and TIMPs -- Assay of MMP and TIMP activities. Mmps and timps. An historical perspective / J.F. Woessner, Jr. -- Strategies for cloning new mmps and timps / G. Velasco and C. Lopez-Otin -- Structural studies on mmps and timps / W. Bode and K. Maskos -- Matrix metalloproteinase substrate binding domains, modules and exosites. Overview and experimental strategies / C.M. Overall -- The matrix metalloproteinase (mmp) and tissue inhibitor of metalloproteinase (timp) genes. Transcriptional and posttranscriptional regulation, signal transduction and cell-type-specific expression / M.P. Vincenti -- Models for gain-of-function and loss-of-function of mmps. Transgenic and gene targeted mice / L.M. Coussens ... [et al.] -- Expression of mmps and timps in mammalian cells / K.M. Yeow ... [et al.] -- Expression of recombinant matrix metalloproteinases in escherichia coli / L.J. Windsor and D.L. Steele -- Expression of human collagenase i (mmp-1) and timp-1 in a baculovirus-based expression system / R. Vallon and P. Angel -- Expression of recombinant matrix metalloproteinases in yeast / G.A. Doyle -- Expression of recombinant membrane-type mmps / M.J. Butler, M.P. d'Ortho and S.J. Atkinson -- Refolding of timp-2 from escherichia coli inclusion bodies / R.A. Williamson -- Expression and refolding of full-length human timp-1 / D. Davis -- Purification of mmps and timps / K. Shimokawa and H. Nagase -- Monitoring mmp and timp mrna expression by rt-pcr / H. Wong ... [et al.] -- Measuring transcription of metalloproteinase genes. Nuclear run-off assay vs analysis of hnrna / A.M. Delany -- In situ hybridization for metalloproteinases and their inhibitors / T.L. Hurskainen and S.S. Apte -- Use of eia to measure mmps and timps / N. Fujimoto and K. Iwata -- Immunohistochemistry of mmps and timps / Y. Okada -- Detecting polymorphisms in mmp genes / S. Ye and A.M. Henney -- Methods for studying activation of matrix metalloproteinases / V. Knauper and G. Murphy -- Assay of matrix metalloproteinases against matrix substrates / T.E. Cawston, P. Koshy and A.D. Rowan -- Zymography and reverse zymography for detecting mmps, and timps / S.P. Hawkes, H. Li and G.T. Taniguchi -- In situ zymography / S.J. George and J.L. Johnson -- Detection of focal proteolysis using texas-red-gelatin / R.M. Hembry -- Antibodies to mmp-cleaved aggrecan / A.J. Fosang ... [et al.] -- Cartilage proteoglycan release assay / C.J. Billington -- Immunoassay for collagenase-mediated cleavage of types i and ii collagens / R.C. Billinghurst, M. Ionescu and A.R. Poole -- Collagen degradation assays / A.P. Hollander -- Invasion assays and matrix metalloproteinases. Quantification of cellular invasion using propidium iodide labeling and confocal laser scanning microscopy / U. Benbow, K.A. Orndorff and A.L. Givan -- Using fluorogenic peptide substrates to assay matrix metalloproteinases / G.B. Fields -- Kinetic analysis of the inhibition of matrix metalloproteinases by tissue inhibitor of metalloproteinases (timp) / M. Hutton and F. Willenbrock -- Assaying growth factor activity of tissue inhibitors of metalloproteinases / K. Yamashita and T. Hayakawa.