Kinesin protocols

Purification of kinesin from the brain / Sergei A. Kuznetsov, Vladimir I. Gelfand -- RT-PCR for the identification of developmentally regulated novel members of the kinesisn-like superfamily / Niovi Santama -- Expression cloning with pan kinesin antibodies / Laura M. Ginkel, Linda Wordeman -- Expression of kinesin in Escherichia coli / Maryanne F. Stock, David D. Hackney -- Plasmids for expression of chimeric and truncated kinesin proteins / Kimberly W. Waligora, Sharyn A. Endow -- Preparation of recombinent kinesin superfamily proteins using the baculovirus system / Nobutaka Hirokawa, Yasuko Noda -- Assays for kinesin microtubule-stimulated ATPase activity / David D. Hackney, Wei Jiang -- An improved microscope for bead and surface-based motility assays / Nick Carter, Rob Cross -- Use of photonic force microscopy to study single-motor molecule mechanics / Sylvia Jeney, Ernst-Ludwig Florin, J.K. Heinrich Hörber -- Assays for microtubule-destabilizing kinesins / Arshad Desai, Claire E. Walczak -- Green fluorescent protein as a tag for molecular motor proteins / Sharyn A. Endow -- In vitro reconstitution of endosome motility along microtubules / Erik Nielsen ... [et al.] -- Approaches to study interactiions between kinesin motors and membranes / Gerardo Morfini ... [et al.] -- Microinjection methods for analyzing the functions of kinesin in early embryos / Robert Le Morris ... [et al.] -- The use of dominant negative mutants to study the function of mitotic motors in the in vitro spindle assembly assay in Xenopus egg extracts / Haralabia Boleti, Eric Karsenti, Isabelle Vernos -- A dominant negative approach for functional studies of the kinesin II complex / Vladimir I. Gelfand ... [et al.] -- Identification of kinesin-associated proteins / Lisa C. Lindesmith, Jamardan Kumar, Michael P. Sheetz -- Assaying spatial organization of microtubules by kinesin motors / Francois Nédélec, Thomas Surrey -- Crystallization of kinesin / Manfred Thormählen, Jens Müller, Eckard Mandelkow -- Structural analysis of the microtubule-kinesin complex by cryo-electron microscopy / Fabienne Bevron, Andreas Hoenger