Methods in biological oxidative stress

1 Measurement of Fat-Soluble Vitamins and Antioxidants by HPLC With Electrochemical Array Detection Paul H. Gamache, Paul A. Ullucci, Joe A. Archangelo, and Ian N. Acworth ...................................................................... 3 2 Analysis of Aldehydic Markers of Lipid Peroxidation in Biological Tissues by HPLC With Fluorescence Detection Mark A. Lovell and William R. Markesbery ............................. 17 3 Measurement of Isofurans by Gas Chromatography– Mass Spectrometry/Negative Ion Chemical Ionization Joshua P. Fessel and L. Jackson Roberts, II ............................... 23 4 Analysis of F2-Isoprostanes by Gas Chromatography–Mass Spectrometry/Negative Ion Chemical Ionization L. Jackson Roberts, II and Jason D. Morrow............................. 33 5 Measurement of F4-Neuroprostanes by Gas Chromatography– Mass Spectrometry/Negative Ion Chemical Ionization Nathalie Bernoud-Hubac and L. Jackson Roberts, II .............. 41 6 Immunoassays for Lipid Peroxidation End Products: One-Hour ELISA for Protein-Bound Acrolein and HNE Kimihiko Satoh and Koji Uchida ................................................ 49 7 Fluorometric and Colorimetric Assessment of Thiobarbituric Acid-Reactive Lipid Aldehydes in Biological Matrices Kelly S. Williamson, Kenneth Hensley, and Robert A. Floyd .... 57 8 HPLC With Electrochemical and Photodiode Array Detection Analysis of Tocopherol Oxidation and Nitration Products in Human Plasma Kelly S. Williamson, Kenneth Hensley, and Robert A. Floyd .... 67PART II DNA, PROTEIN, AND AMINO ACIDS 9 Electron Paramagnetic Resonance Spin-Labeling Analysis of Synaptosomal Membrane Protein Oxidation D. Allan Butterfield ........................................................................ 79 10 Gas Chromatography–Mass Spectrometric Analysis of Free 3-Chlorotyrosine, 3-Bromotyrosine, Ortho-Tyrosine, and 3-Nitrotyrosine in Biological Fluids Joseph P. Gaut, Jaeman Byun, and Jay W. Heinecke ............... 87 11 Isotope Dilution Gas Chromatography–Mass Spectrometric Analysis of Tyrosine Oxidation Products in Proteins and Tissues Jay W. Heinecke ............................................................................... 93 12 Single-Cell Gel Electrophoresis or Comet Assay of Intestinal Epithelial Cells Using Manual Scoring and Ridit Analysis Mark M. Huycke ............................................................................ 101 13 Detection of Aldehydic DNA Lesions Using Aldehyde Reactive Probe Jun Nakamura and James A. Swenberg .................................... 109 14 Analysis of Neuroketal Protein Adducts by Liquid Chromatography–Electrospray Ionization/Tandem Mass Spectrometry Nathalie Bernoud-Hubac, Sean S. Davies, Olivier Boutaud, and L. Jackson Roberts, II......................................................... 117 15 Measurement of Isoketal Protein Adducts by Liquid Chromatography–Electrospray Ionization/Tandem Mass Spectrometry Sean S. Davies, Cynthia J. Brame, Olivier Boutaud, Nathalie Bernoud-Hubac, and L. Jackson Roberts, II ....... 127 16 Bioassay of 2
-Deoxyguanosine/8-Hydroxy-2
- Deoxyguanosine by HPLC With Electrochemical/ Photodiode Array Detection Kelly S. Williamson, Kenneth Hensley, Quentin N. Pye, Scott Ferrell, and Robert A. Floyd ......................................... 137 17 HPLC With Electrochemical Detection Analysis of 3-Nitrotyrosine in Human Plasma Kelly S. Williamson, Kenneth Hensley, and Robert A. Floyd ... 151PART III REACTIVE OXYGEN SPECIES AND REACTIVE NITROGEN SPECIES 18 Protein Carbonyl Levels—An Assessment of Protein Oxidation Alessandra Castegna, Jennifer Drake, Chava Pocernich, and D. Allan Butterfield .......................................................... 161 19 Fluorogenic Analysis of H2O2 in Biological Materials Kenneth Hensley, Kelly S. Williamson, and Robert A. Floyd ... 169 20 Detection of Reactive Oxygen Species by Flow Cytometry Alexander Christov, Ladan Hamdheydari, and Paula Grammas ............................................................... 175 21 Nitrite Determination by Colorimetric and Fluorometric Greiss Diazotization Assays: Simple, Reliable, High-Throughput Indices of Reactive Nitrogen Species in Cell-Culture Systems Kenneth Hensley, Shenyun Mou, and Quentin N. Pye ........... 185 22 Protein Carbonyl Determination Using Biotin Hydrazide Kenneth Hensley and Kelly S. Williamson .............................. 195 23 Real-Time, In-Vivo Measurement of Nitric Oxide Using Electron Paramagnetic Resonance Spectroscopic Analysis of Biliary Flow Kenneth Hensley, Yashige Kotake, Danny R. Moore, Hong Sang, and Lester A. Reinke ........................................... 201