Two-hybrid systems : methods and protocols

Part I. General Methods -- The Two-Hybrid System : A Personal View / Stanley Fields and Paul L. Bartel -- Growth and Maintenance of Yeast / Lawrence W. Bergman -- Media Formulations for Various Two-Hybrid Systems / Michael Saghbini, Denise Hoekstra, and Jim Gautsch -- Yeast Two-Hybrid Vectors and Strains / Philip James -- High-Efficiency Transformation of Plasmid DNA into Yeast / Robin A. Woods and R. Daniel Gietz -- Qualitative and Quantitative Assessment of Interactions / Monica M. Montano -- Strategies for Rescuing Plasmid DNA from Yeast Two-Hybrid Colonies / Alyson Byrd and René St-Arnaud -- Part II. False Positives -- Two-Hybrid System and False Positives : Approaches to Detection and Elimination / Ilya G. Serebriiskii and Erica A. Golemis -- Confirming Yeast Two-Hybrid Protein Interactions Using In Vitro Glutathione-S-Transferase Pulldowns / Dennis M. Kraichely and Paul N. MacDonald -- Two-Hybrid Interactions Confirmed by Coimmunoprecipitation of Epitope-Tagged Clones / Louie Naumovski -- Part III. Applications -- Smad Interactors in Bone Morphogenetic Protein Signaling / Xiangli Yang and Xu Cao -- Protein Interactions Important in Eukaryotic Translation Initiation / Katsura Asano and Alan G. Hinnebusch -- Steroid Receptor and Ligand-Dependent Interaction with Coactivator Proteins / Sergio A. Oñate -- Interaction of Cellular Apoptosis Regulating Proteins with Adenovirus Anti-apoptosis Protein EB-K / Thirugnana Subramanian and G. Chinnadurai -- Part IV. Alternative Strategies -- Mammalian Two-Hybrid Assays : Analyzing Protein-Protein Interactions in the Transforming Growth Factor-[beta] Signaling Pathway / Xin-Hua Feng and Rik Derynck -- One-Hybrid Systems for Detecting Protein-DNA Interactions / Mary Kate Alexander, Brenda D. Bourns, and Virginia A. Zakian -- The Split-Hybrid System : Uncoding Multiprotein Networks and Defining Mutations That Affect Protein Interactions / Phyllis S. Goldman ... [et al.] -- Three-Hybrid Screens : Inducible Third-Party Systems / Björn Sandrock, Franck Tirode, and Jean-Marc Egly -- Three-Hybrid Screens for RNA-Binding Proteins : Proteins Binding 3' End of Histone mRNA / Zbigniew Dominski and William F. Marzluff -- Membrane Recruitment Systems for Analysis of Protein-Protein Interactions / Ami Aronheim.