RT-PCR protocols

Rt-pcr in biomedicine. Opportunities arising from the new accessibility of mrna / J. O'Connell -- The basics of rt-pcr. Some practical considerations / J. O'Connell -- Using the quantitative competitive rt-pcr technique to analyze minute amounts of different mrnas in small tissue samples / S. Greber-Platzer ... [et al.] -- Detection of mrna expression and alternative splicing in a single cell / T. Kumazaki -- Nested rt-pcr. Sensitivity controls are essential to determine the biological significance of detected mrna / T. Goode ... [et al.] -- Quantitative rt-pcr. A review of current methodologies / C. Joyce -- Rapid development of a quantitative-competitive (qc) rt-pcr assay using a composite primer approach / J. O'Connell ... [et al.] -- Quantitation of gene expression by rt-pcr and hplc analysis of pcr products / F. Bachmair, C.G. Huber and G. Daxenbichler -- Time-resolved fluorometric detection of cytokine mrnas amplified by rt-pcr / K. Nieminen ... [et al.] -- Mimic-based rt-pcr quantitation of substance p mrna in human mononuclear phagocytes and lymphocytes / J.P. Lai, S.D. Douglas and W.Z. Ho -- Detection and quantification of the hepatitis c viral genome / L.J. Fanning -- Semi-quantitative detection of hepatitis c virus rna by "real-time" rt-pcr / J.F. Schlaak -- Rt-pcr for the assessment of genetically heterogenous populations of the hepatitis c virus / B. Mullan ... [et al.] -- In situ immuno-pcr. A newly developed method for highly sensitive antigen detection in situ / Y. Cao -- Rt-pcr from laser-capture microdissected samples / T. Crnogorac-Jurcevic, T.O. Nielsen and N.R. Lemoine -- Mycobacterium paratuberculosis detected by nested pcr in intestinal granulomas isolated by lcm in cases of crohn's disease / P. Ryan ... [et al.] -- Rt-pcr-based approaches to generate probes for mrna detection by in situ hybridization / J. O'Connell -- Amplified rna for gene array hybridizations / V.I. Shustova and S.J. Meltzer -- Semi-quantitative determination of differential gene expression in primary tumors and matched metastases by rt-pcr. Comparison with other methods / B. Mann and C. Hanski -- Detection of single nucleotide polymorphisms using a non-isotopic rnase cleavage assay / F. Waldron-Lynch ... [et al.] -- Detection of clonally expanded t-cells by rt-pcr-sscp and nucleotide sequencing of t-cell receptor beta-cdr3 regions / M.S. Kurokawa, K. Nishioka and T. Kato -- Generation of scfv from a phage display mini-library derived from tumor-infiltrating b-cells / N. Gruel ... [et al.] -- Generation of murine scfv intrabodies from b-cell hybridomas / C.H. Nam, S. Moutel and J.L. Teillaud -- Quantitation of mrna levels by rt-pcr in cells purified by facs. Application to peripheral cannabinoid receptors in leukocyte subsets / J. Marchand and P. Carayon -- Detection of anti-sense rna transcripts by anti-sense rt-pcr / M.C. Yeung and A.S. Lau -- Rt-pcr in cdna library construction / V. Healy -- An rt-pcr-based protocol for the rapid generation of large, representative cdna libraries for expression screening / J. O'Connell.