In vitro mutagenesis protocols

Rapid and Reliable Site-Directed Mutagenesis Using Kunkel's Approach / Priya Handa, Swapna Thanedar, and Umesh Varshney -- Site-Directed Mutagenesis Using Altered b-Lactamase Specificity / Christine A. Andrews and Scott A. Lesley -- Site-Directed Mutagenesis Facilitated by DpnI Selection on Hemimethylated DNA / Fusheng Li and James I. Mullins -- Multiple Site-Directed Mutagenesis In Vitro / Yang-Gyun Kim and Stefan Maas -- Two-Stage Polymerase Chain Reaction Protocol Allowing Introduction of Multiple Mutations, Deletions, and Insertions, Using QuikChange Site-Directed Mutagenesis / Wenyan Wang and Bruce A. Malcolm -- Efficient and Accurate Site-Directed Mutagenesis of Large Plasmids / Susan A. Nadin-Davis -- Combining Site-Specific Chemical Modification with Site-Directed Mutagenesis : Versatile Strategy to Move Beyond Structural Limitations of 20 Natural Amino Acids Side Chains in Protein Engineering / Grace DeSantis and J. Bryan Jones -- Site-Directed Mutagenesis Mediated by a Single Polymerase Chain Reaction Product / Xueni Chen ... [et al.] -- Megaprimer Method for Polymerase Chain Reaction-Mediated Generation of Specific Mutations in DNA / Jesper Brons-Poulson, Jane Nohr, and Leif Kongskov Larsen -- Generation of Epitope-Tagged Proteins by Inverse Polymerase Chain Reaction Mutagenesis / Lucio Gama and Gerda E. Breitwieser -- Site-Directed Mutagenesis by Polymerase Chain Reaction / Albert Jeltsch and Thomas Lanio -- Generation of Multiple Site-Specific Mutations by Polymerase Chain Reaction / Amom Ruhikanta Meetei and M.R.S. Rao -- Phenotypic Expression of Polymerase Chain Reaction-Generated Random Mutations in a Foreign Gene After its Introduction into an Acinetobacter Chromosome by Natural Transformation / David M. Young, Ruben G. Kok, and L. Nicholas Ornston -- Polymerase Chain Reaction-Mediated Mutagenesis in Sequences Resistant to Homogeneous Amplification / Ross N. Nazar, P.D. Abeyrathne, and Robert V.A. Intine -- Polymerase Chain Reaction-Based Signature-Tagged Mutagenesis / Dario E. Lehoux and Roger C. Levesque -- High-Throughput Scanning Mutagenesis by Recombination Polymerase Chain Reaction / Stefan Howorka and Hagan Bayley -- In Vitro Scanning-Saturation Mutagenesis / Jennifer A. Maynard ... [et al.] -- Random Transposon Mutagenesis of Large DNA Molecules in Escherichia coli / Wolfram Brune -- Random Chromosomal Gene Disruption Using Cassette Mutagenesis / French A. Lewis, III and Brian Dougherty -- Transplacement Mutagenesis : A Recombination-Based In Situ Mutagenesis Protocol / Knut Woltjen, M.W. Todd Unger, and Derrick E. Rancourt -- Preparation of Transposon Insertion Lines and Determination of Insertion Sites in Arabidopsis Genome / Takuya Ito, Reiko Motohashi, and Kazuo Shinozaki -- Evolutionary Molecular Engineering by Random Elongation Mutagenesis / Tomoaki Matsuura, Tetsuya Yomo, and Itaru Urabe -- Random Mutagenesis for Protein Breeding / Chris Fenton ... [et al.] -- DNA Shuffling and Family Shuffling for In Vitro Gene Evolution / Miho Kikuchi and Shigeaki Harayama -- Mutagenic Polymerase Chain Reaction of Protein-Coding Genes for In Vitro Evolution / Ichiro Matsumura and Andrew D. Ellington.