In situ detection of DNA damage : methods and protocols

pt. 1. Labeling DNA breaks using terminal transferase (TUNEL assay) -- Labeling DNA damage with terminal transferase: applicability, specificity, and limitations / P. Roy Walker -- TUNEL assay: an overview of techniques / Deryk T. Loo -- Electron microscopic detection of DNA damage labeled by TUNEL / Antonio Migheli -- Quanitative differentiation of both free 3' OH and 5' OH DNA ends using terminal transferase-based labeling combined with transmission electron microscopy / Yoshinori Otsuki -- Determination of three-dimensional distribution of apoptotic DNA damage by combination of TUNEL and quick-freezing and deep-etching techniques / Shinichi Ohno -- In situ detection of DNA strand breaks in analysis of apoptosis by flow- and laser-scanning cytometry / Zbigniew Darzynkiewicz -- pt. 2. Labeling DNA breaks using DNA polymerase I or its Klenow fragment -- DNA damage detection using DNA polymersase I or its Klenow fragment: applicability, specificity, limitations / Jan Hein van Dierendonck -- Labeling DNA breaks in situ by Klenow enzyme / Katherine A. Wood -- In situ nick translation at the electron microscopic level / Marc Thiry -- pt. 3. Labeling DNA breaks using ligase -- In situ DNA ligation as a method for labeling apoptotic cells in tissue sections: an overview / Peter J. Hornsby -- Detection of specific double-strand DNA breaks and apoptosis in situ using T4 DNA ligase / Vladimir V. Didenko -- In situ detection of double-strand DNA breaks with terminal 5'OH groups / Vladimir V. Didenko -- pt. 4. Detection of DNA breaks in agarose trapped cells: comet assay and related techniques -- The comet assay: principles, applications, and limitations / Andrew R. Collins -- The comet assay: an overview of techniques / Peggy L. Olive -- Ultrasensitive detection of DNA damage by the combination of the comet and TUNEL assays / Andrei L. Kindzelskii -- Application of FISH to detect DNA damage: DNA breakage detection-FISH (DBD-FISH) / José Luis Fernández -- pt. 5. Detection of modified bases and AP sites in DNA -- Simultaneous in situ detection of DNA fragmentation and RNA/DNA oxidative damage using TUNEL assay and immunohistochemical labeling for 8-hydroxy-2'-deoxyguanosine (8-OhdG) / Alexander E. Kalyuzhny -- The in situ detection of apurinic/apyrimidinic sites and DNA breaks bearing extension blocking termini / Philip K. Liu -- pt. 6. Indirect and general markers of DNA damage -- Markers of poly (ADP-ribose) polymerase activity as correlates of DNA damage / Yinong Zhou -- Ultrasound imaging of apoptosis: DNA-damage effects visualized / Gregory J. Czarnota -- p53 induction as an indicator of DNA damage / Galina Selivanova -- -Detection of caspases activation in situ by fluorochrome-labeled inhibitors of caspases (FLICA) / Zbigniew Darzynkiewicz