Neural stem cells

Preface .............................................................................................................v Contributors ..................................................................................................... xi PART I ISOLATION AND CULTURE OF NSCS 1 Neural Differentiation of Embryonic Stem Cells K. Sue O’Shea ........................................................................................ 3 2 Production and Analysis of Neurospheres from Acutely Dissociated and Postmortem CNS Specimens Eric D. Laywell, Valery G. Kukekov, Oleg Suslov, Tong Zheng, and Dennis A. Steindler........................................... 15 3 Isolation of Stem and Precursor Cells from Fetal Tissue Yuan Y. Wu, Tahmina Mujtaba, and Mahendra S. Rao ................... 29 4 Olfactory Ensheathing Cells: Isolation and Culture from the Rat Olfactory Bulb Susan C. Barnett and A. Jane Roskams ........................................... 41 5 Culturing Olfactory Ensheathing Glia from the Mouse Olfactory Epithelium Edmund Au and A. Jane Roskams .................................................... 49 6 Production of Immortalized Human Neural Crest Stem Cells Seung U. Kim, Eiji Nakagawa, Kozo Hatori, Atsushi Nagai, Myung A. Lee, and Jung H. Bang .................................................. 55 7 Adult Rodent Spinal Cord Derived Neural Stem Cells: Isolation and Characterization Lamya S. Shihabuddin ........................................................................ 67 8 Preparation of Neural Progenitors from Bone Marrow and Umbilical Cord Blood Shijie Song and J. Sanchez-Ramos .................................................. 79 9 Seeding Neural Stem Cells on Scaffolds of PGA, PLA, and Their Copolymers Erin Lavik, Yang D. Teng, Evan Snyder, and Robert Langer ......... 89 vii viii Contents PART II CHARACTERIZATION OF NSCS IN VITRO A. CELLULAR TECHNIQUES 10 Analysis of Cell Generation in the Telencephalic Neuroepithelium Takao Takahashi, Verne S. Caviness, Jr., and Pradeep G. Bhide ................................................................... 101 11 Clonal Analyses and Cryopreservation of Neural Stem Cell Cultures Angelo L. Vescovi, Rossella Galli, and Angela Gritti ................... 115 12 Assessing the Involvement of Telomerase in Stem Cell Biology Mark P. Mattson, Peisu Zhang, and Weiming Fu ........................... 125 13 Detection of Telomerase Activity in Neural Cells Karen R. Prowse ................................................................................ 137 14 In Vitro Assays for Neural Stem Cell Differentiation Marcel M. Daadi .................................................................................. 149 15 Electron Microscopy and Lac-Z Labeling Bela Kosaras and Evan Snyder ....................................................... 157 B. ELECTROPHYSIOLOGICAL TECHNIQUES 16 Techniques for Studying the Electrophysiology of Neurons Derived from Neural Stem/Progenitor Cells David S. K. Magnuson and Dante J. Morassutti ............................ 179 C. MOLECULAR TECHNIQUES 17 Fluorescence In Situ Hybridization Barbara A. Tate and Rachel L. Ostroff ............................................ 189 18 RT-PCR Analyses of Differential Gene Expression in ES-Derived Neural Stem Cells Theresa E. Gratsch ............................................................................ 197 19 Differential Display: Isolation of Novel Genes Theresa E. Gratsch ............................................................................ 213 20 Cell Labeling and Gene Misexpression by Electroporation Terence J. Van Raay and Michael R. Stark .................................... 223 21 Gene Therapy Using Neural Stem Cells Luciano Conti and Elena Cattaneo.................................................. 233 22 Modeling Brain Pathologies Using Neural Stem Cells Simonetta Sipione and Elena Cattaneo .......................................... 245 PART III UTILIZATION/CHARACTERIZATION OF NSCS IN VIVO A. ENDOGENOUS POOLS OF STEM/PROGENITOR CELLS 23 Activation and Differentiation of Endogenous Neural Stem Cell Progeny in the Rat Parkinson Animal Model Marcel M. Daadi .................................................................................. 265 24 Identification of Musashi1-Positive Cells in Human Normal and Neoplastic Neuroepithelial Tissues by Immunohistochemical Methods Yonehiro Kanemura, Shin-ichi Sakakibara, and Hideyuki Okano ...................................................................... 273 25 Identification of Newborn Cells by BrdU Labeling and Immunocytochemistry In Vivo Sanjay S. P. Magavi and Jeffrey D. Macklis ................................... 283 26 Immunocytochemical Analysis of Neuronal Differentiation Sanjay S. P. Magavi and Jeffrey D. Macklis ................................... 291 27 Neuroanatomical Tracing of Neuronal Projections with Fluoro-Gold Lisa A. Catapano, Sanjay S. P. Magavi, and Jeffrey D. Macklis .................................................................. 299 B. TRANSPLANTATION 28 Labeling Stem Cells In Vitro for Identification of Their Differentiated Phenotypes After Grafting into the CNS Qi-lin Cao, Stephen M. Onifer, and Scott R. Whittemore ............. 307 29 Optimizing Stem Cell Grafting into the CNS Scott R. Whittemore, Y. Ping Zhang, Christopher B. Shields, Dante J. Morassutti, and David S. K. Magnuson ....................... 319 30 Vision-Guided Technique for Cell Transplantation and Injection of Active Molecules into Rat and Mouse Embryos Lorenzo Magrassi .............................................................................. 327 31 Transplantation into Neonatal Rat Brain as a Tool to Study Properties of Stem Cells Tanja Zigova and Mary B. Newman ................................................. 341 32 Routes of Stem Cell Administration in the Adult Rodent Alison E. Willing, Svitlana Garbuzova-Davis, Paul R. Sanberg, and Samuel Saporta ....................................... 357 Index ............................................................................................................ 375