E. coli gene expression protocols

1. Cold-Inducible Promoters for Heterologous Protein Expression / Francois Baneyx and Mirna Mujacic -- 2. Dual-Expression Vectors for Efficient Protein Expression in Both E. coil and Mammalian Cells / Rebecca L. Mullinax ... [et al.] -- 3. A Dual-Expression Vector Allowing Expression in E: coli and P. pastoris, Including New Modifications / Angelika Lueking ... [et al.] -- 4. Purification of Recombinant Proteins from E. coli by Engineered Inteins / Ming-Qun Xu and Thomas C. Evans, Jr. -- 5. Calmodulin as an Affinity Purification Tag / Samu Melkko and Dario Neri -- 6. Calmodulin-Binding Peptide as a Removable Affinity Tag for Protein Purification / Wolfgang Klein -- 7. Maltose-Binding Protein as a Solubility Enhancer / Jeffrey D. Fox and David S. Waugh -- 8. Thioredoxin and Related Proteins as Multifunctional Fusion Tags for Soluble Expression in E. coli / Edward R. LaVallie ... [et al.] -- 9. Discovery of New Fusion Protein Systems Designed to Enhance Solubility in E. coli / Gregory D. Davis and Roger G. Harrison -- 10. Assessment of Protein Folding/Solubility in Live Cells / Rhesa D. Stidham ... [et al.] -- 11. Improving Heterologous Protein Folding via Molecular Chaperone and Foldase co-Expression / Francois Baneyx and Joanne L. Palumbo -- 12. High-Throughput Purification of PolyHis-Tagged Recombinant Fusion Proteins / Thomas Lanio, Albert Jeltsch, and Alfred Pingoud -- 13. Co-Expression of Proteins in E. coil Using Dual Expression Vectors / Karen Johnston and Ronen Marmorstein -- 14. Small-Molecule Affinity-Based Matrices for Rapid Protein Purification / Karin A. Hughes and Jean P. Wiley -- 15. Use of tRNA-Supplemented Host Strains for Expression of Heterologous Genes in E. coli / Carsten-Peter Carstens -- 16. Screening Peptide/Protein Libraries Fused to the X Repressor DNA-Binding Domain in E. coil Cells / Leonardo Marino-Ramirez, Lisa Campbell, and James C. Hu -- 17. Studying Protein-Protein Interactions Using a Bacterial Two-Hybrid System / Simon L. Dove -- 18. Using Bio-Panning of FLITRX Peptide Libraries Displayed on E. coli Cell Surface to Study Protein-Protein Interactions / Zhijian Lu, Edward R. LaVallie, and John M. McCoy -- 19. Use of Inteins for the In Vivo Production of Stable Cyclic Peptide Libraries in E. coli / Ernesto Abel-Santos, Charles P. Scott, and Stephen J. Benkovic -- 20. Hyperphage : Improving Antibody Presentation in Phage Display / Olaf Broders, Frank Breitling, and Stefan Dibel -- 21. Combinatorial Biosynthesis of Novel Carotenoids in E. coli / Gerhard Sandmann -- 22. Using Transcriptional-Based Systems for In Vivo Enzyme Screening / Steven M. Firestine, Frank Salinas, and Stephen J. Benkovic -- 23. Identification of Genes Encoding Secreted Proteins Using Mini-OphoA Mutagenesis / Mary N. Burtnick, Paul J. Brett, and Donald E. Woods.