Bone research protocols

Human osteoblast culture / J.A. Gallagher -- Osteoblast isolation from murine calvariae and long bones / A. Bakker and J. Klein-Nulend -- Mineralizing fibroblast-colony-forming assays / A. Scutt ... [et al.] -- Osteocyte isolation and culture / P.J. Nijweide ... [et al.] -- Isolated osteoclast cultures / A. Hoebertz and T.R. Arnett -- Primary isolation and culture of chicken osteoclasts / P. Collin-Osdoby, F. Anderson and P. Osdoby -- Isolation and purification of rabbit osteoclasts / F.P. Coxon ... [et al.] -- Generating human osteoclasts from peripheral blood / A. Sabokbar and N.S. Athanasou -- Generating human osteoclasts in vitro from bone marrow and peripheral blood / A.M. Flanagan and H.M. Massey -- Generating murine osteoclasts from bone marrow / N. Takahashi ... [et al.] -- Osteoclast formation in the mouse coculture assay / R.J. van 't Hof -- RANKL-mediated osteoclast formation from murine RAW 264.7 cells / P. Collin-Osdoby ... [et al.] -- Analysis of osteoclast function in mouse calvarial cultures / M.J. Marshall and M.N. Rowlands -- Assessing bone formation using mouse calvarial organ cultures / I.R. Garrett -- In situ hybridization and in situ reverse transcription polymerase chain reaction in human bone sections / A.P. Mee and J.A. Hoyland -- Techniques for the study of apoptosis in bone / B.S. Noble and H.Y. Stevens -- Protein localization in wax-embedded and frozen sections of bone using immunohistochemistry / S. Bord -- Detection of noncollagenous bone proteins in methylmethacrylate-embedded human bone sections / J.P. van Leeuwen and P. Derkx -- Fluorescence imaging of bone-resorbing osteoclasts by confocal microscopy / S.A. Nesbitt and M.A. Horton -- Bone histomorphometry / S. Vedi and J. Compston -- Transmission electron microscopy of bone / V. Everts, A. Niehof and W. Beertsen -- Scanning electron microscopy of bone / D. Marshall, M.H. Helfrich and R.M. Aspden -- Bone measurements by peripheral quantitative computed tomography in rodents / J.A. Gasser -- Studies of local bone remodeling. The calvarial injection assay / R.J. van 't Hof, C.E. Clarkin and K.J. Armour -- Inflammation-induced osteoporosis. The IMO model / K.J. Armour and K.E. Armour -- Ovariectomy and estrogen replacement in rodents / J.W. Chow -- Mechanical testing of bone ex vivo / R.M. Aspden -- Bone cell responses to fluid flow / H.Y. Stevens and J.A. Frangos -- Methods for analyzing bone cell responses to mechanical loading using in vitro monolayer and organ culture models / A.A. Pitsillides ... [et al.] -- Extraction of nucleic acids from bone / T.L. Stewart and V. Mann -- Analysis of gene expression in bone by quantitative RT-PCR / D. Ireland