Transgenic plants : methods and protocols

Transgenic plants: an historical perspective / Luis Herrera-Estrella, June Simpson, and Miguel Martínez-Trujillo -- Plant transformation: agrobacterium-mediated gene transfer / Abhaya M. Dandekar and Henry J. Fisk -- Production of hairy root cultures and transgenic plants by Agrobacterium rhizogenes-mediated transformation / Mary C. Christey and Robert H. Braun -- Stable transformation of plant cells by particle bombardment/biolistics /Julie R. Kikkert, José R. Vidal, and Bruce I. Reisch -- Electroporation: introduction and expression of transgenes in plant protoplasts / Henry J. Fisk and Abhaya M. Dandekar -- Floral dip: agrobacterium-mediated germ line transformation / Steven J. Clough -- Production of transgenic crops by the floral-dip method / Ian S. Curtis -- Chloroplast genetic engineering to improve agronomic traits / Henry Daniell, Oscar N. Ruiz, and Amit Dhingra -- Organogenesis from transformed tomato explants / Anne Frary and Joyce Van Eck -- Genetic transformation of conifers utilizing somatic embryogenesis / Krystyna Klimaszewska, Robert G. Rutledge, and Armand Séguin -- Regeneration of transgenic cassava from transformed embryogenic tissues / Peng Zhang and Johanna Puonti-Kaerlas -- Genetic transformation of mature citrus plants / Magdalena Cervera ... [et al.] -- Selectable markers: antibiotic and herbicide resistance / Julia L. Goodwin ... [et al.] -- Histochemical and fluorometric assays for uidA (GUS) gene detection / Magdalena Cervera -- Green fluorescent protein quantification in whole plants / Matthew D. Halfhill, Reginald J. Millwood, and C. Neal Stewart, Jr. -- Positive selection / Allan Wenck and Geneviève Hansen -- Elimination of marker genes from transgenic plants using MAT vector systems / Hiroyasu Ebinuma ... [et al.] -- Simple and efficient removal of marker genes from plastids by homologous recombination / Anil Day ... [et al.] -- The study of transgene copy number and organization / David J. Ingham -- Analysis of gene expression in transgenc plants / Andrew F. Page and Subhash C. Minocha -- Transgene integration: use of matrix attachment regions / George C. Allen, Steven Spiker, and William F. Thompson -- Fluorescence in situ hybridization to localize transgenes in plant chromosomes / Wendy A. Harwood ... [et al.] -- Amplification of genomic sequences flanking T-DNA insertions by thermal asymmetric interlaced polymerase chain reaction / Yao-Guang Liu, Yuanlin Chen, and Qunyu Zhang -- Agrobacterium persistence in plant tissues after transformation / Jaime Cubero and María M. López -- Transgene dispersal through pollen / Laura C. Hudson, Matthew D. Halfhill, and C. Neal Stewart, Jr. -- Transgenic crops: the current and next generations / Jim M. Dunwell -- Comparative development and impact of transgenic papayas in Hawaii, Jamaica, and Venezuela / Gustavo Fermín ... [et al.].