Xenopus protocols : cell biology and signal transduction

Resources for genetic and genomic studies of xenopus -- The physiology of the xenopus laevis ovary -- Oocyte isolation and enucleation -- Xenopus tropicalis oocytes: more than just a beautiful genome -- Oocyte expression with injection of purified T7 RNA polymerase -- Visualization of the cytoskeleton in xenopus ooxytes and eggs by confocal immunofluorescence microscopy -- Multiphoton laser scanning microscopy as a tool for Xenopus oocyte research -- Imaging Ca²⁺ signals in xenopus oocytes -- Chromosomal DNA replication in a soluble cell-free system derived from xenopus eggs -- Chromatin assembly of DNA templates microinjected into xenopus oocytes -- Pre-mRNA splicing in the nuclei of xenopus oocytes -- Chromatin immunoprecipitation for studying transcriptional regulation in xenopus oocytes and tadpoles -- Cytoplasmic mRNA polyadenylation and translation assays. Xenopus egg extracts: a model system to study proprotein convertases -- Analysis of molecular chaperones using a xenopus oocyte protein refolding assay -- Ubiquitin-mediated protein degradation in xenopus egg extracts -- Introduction to nucleocytoplasmic transport: molecules and mechanisms -- Use of xenopus laevis oocyte nuclei and nuclear envelopes in nucleocytoplasmic transport studies -- Nuclear pore complex structure and plasticity revealed by electron and atomic force microscopy -- In vitro study of nuclear assembly and nuclear import using xenopus egg extracts -- Use of intact xenopus oocytes in nculeocytoplasmic transport studies -- Studying the mechanosensitivity of voltage-gated channels using oocyte patches -- Oocytes as an expression system for studying receptor/channel targets of drugs and pesticides -- Microtransplantation of neurotransmitter receptors from cells to xenopus oocyte membranes: new procedure for ion channel studies. Reconstitution of golgi disassembly by mitotic xenopus egg extracts in semi-intact MDCK cells -- Exploring RNA virus replication in xenopus oocytes -- Study of apoptosis in vitro using the xenopus egg extract reconstitution system -- Studying fertilization in cell-free extracts: focusing on membrane/lipid raft functions and proteomics -- Localized sampling, electrophoresis, and biosensor analysis of xenopus laevis cytoplasm for subcellular biochemical assays -- Monitoring protein kinase A activities using expressed substrate in live cells -- Using xenopus oocyte extracts to study signal transduction -- Oocyte extracts for the study of meiotic M-M transition -- Methods for studying spindle assembly and chromosome condensation in xenopus egg extracts