Microbial processes and products

Preface ..............................................................................................................v Contributors .....................................................................................................xi 1. Development of Improved Strains and Optimization of Fermentation Processes Lei Han and Sarad R. Parekh ................................................................ 1 2. Experimental Design in Microbiology Guillaume E. Vanot and Michelle Sergent .......................................... 25 3. Metabolic Engineering of Acremonium chrysogenum to Produce Deacetoxycephalosporin C and Bioconversion to 7-Aminodeacetoxycephalosporanic Acid Marta Rodríguez-Sáiz, Juan-Luis de la Fuente, and José-Luis Barredo ..................................................................... 41 4. Production of Erythromycin With Saccharopolyspora erythraea Wolfgang Minas .................................................................................. 65 5. The Phenylacetyl-CoA Catabolon: Genetic, Biochemical, and Biotechnological Approaches José M. Luengo, Belén García, Ángel Sandoval, Elsa Arias-Barrau, Sagrario Arias, Francisco Bermejo, and Elías R. Olivera ........................................................................ 91 6. Recombinant Microorganisms for the Biosynthesis of Glycosylated Antitumor Compounds Carmen Méndez and José A. Salas ................................................... 131 7. Assay Methods for Detection and Quantification of Antimicrobial Metabolites Produced by Streptomyces clavuligerus Paloma Liras and Juan F. Martín ....................................................... 149 8. Purification of Plasmid DNA Vectors Produced in Escherichia coli for Gene Therapy and DNA Vaccination Applications Maria Margarida Diogo, João António Queiroz, and Duarte Miguel F. Prazeres ..................................................... 165 9. Genome Breeding of an Amino Acid-Producing Corynebacterium glutamicum Mutant Masato Ikeda, Junko Ohnishi, and Satoshi Mitsuhashi .................... 179 viii Contents 10. Metabolic Activity Profiling by 13C Tracer Experiments and Mass Spectrometry in Corynebacterium glutamicum Christoph Wittmann and Elmar Heinzle .......................................... 191 11. Protein and Vitamin Production in Bacillus megaterium Heiko Barg, Marco Malten, Martina Jahn, and Dieter Jahn ............. 205 12. Strategies for Large-Scale Production of Recombinant Proteins in Filamentous Fungi Heidi Sisniega, José-Luis del Río, María-José Amaya, and Ignacio Faus ........................................................................... 225 13. Strain and Culture Conditions Improvement for β-Carotene Production With Mucor Enrique A. Iturriaga, Tamás Papp, Jesper Breum, José Arnau, and Arturo P. Eslava ..................................................................... 239 14. Xanthophylls in Fungi: Metabolic Engineering of the Astaxanthin Biosynthetic Pathway in Xanthophyllomyces dendrorhous Hans Visser, Gerhard Sandmann, and Jan C. Verdoes ..................... 257 15. Methodologies for the Analysis of Fungal Carotenoids Paul D. Fraser and Peter M. Bramley ............................................... 273 16. Insertional Mutagenesis in the Vitamin B2 Producer Fungus Ashbya gossypii María A. Santos, Laura Mateos, Karl-Peter Stahmann, and José-Luis Revuelta .................................................................. 283 17. Improved Polysaccharide Production Using Strain Improvement Thomas P. West ................................................................................ 301 18. Use of the Morganella morganii phoC Gene as Reporter in Bacterial and Yeast Hosts Stefania Cresti, Cesira L. Galeotti, Serena Schippa, Gian Maria Rossolini, and Maria C. Thaller ................................. 313 19. Gene Expression Arrays in Food Bart Weimer, Yi Xie, Lan-szu Chou, and Adele Cutler .................... 333 20. Optimization of Proteome Analysis for Wine Yeast Strains Tammi L. Olineka, Apostolos Spiropoulos, Paula A. Mara, and Linda F. Bisson ....................................................................... 345 21. Bacteriocin-Producing Strains in a Meat Environment Frédéric Leroy and Luc De Vuyst ..................................................... 369 22. In Vitro and In Vivo Interactions of Nonpathogenic Bacteria With Immunocompetent Cells Eduardo J. Schiffrin, Nabila Ibnou-Zekri, Jean M. Ovigne, Thierry von der Weid, and Stephanie Blum ................................. 381 23. Volatile Sulfur Detection in Fermented Foods Bart Weimer and Ben Dias ............................................................... 397 24. Bioleaching: Analysis of Microbial Communities Dissolving Metal Sulfides Axel Schippers and Klaus Bosecker .................................................. 405 25. Use of PhoN Phosphatase to Remediate Heavy Metals Marion Paterson-Beedle and Lynne E. Macaskie .............................. 413 26. Safe Dispatch and Transport of Biological Material Vera Weihs and Christine Rohde ...................................................... 437 27. How to Deposit Biological Material for Patent Purposes Vera Weihs ........................................................................................ 451 28. Laws and Regulations for the Protection of Biotechnological Inventions Uwe Fitzner ...................................................................................... 465 Index ............................................................................................................ 495