Embryonic Stem Cell Protocols : Volume 1: Isolation and Characterization

Preface ..............................................................................................................v Contents of the Companion Volume ................................................................xi Contributors .................................................................................................... xv Guide to the Companion CD ......................................................................... xxi PART I. ISOLATION AND MAINTENANCE 1 Isolation and Differentiation of Medaka Embryonic Stem Cells Yunhan Hong and Manfred Schartl ...................................................... 3 2 Maintenance of Chicken Embryonic Stem Cells In Vitro Hiroyuki Horiuchi, Shuichi Furusawa, and Haruo Matsuda ........................................................................ 17 3 Derivation and Culture of Mouse Trophoblast Stem Cells In Vitro Satoshi Tanaka .................................................................................... 35 4 Derivation, Maintenance, and Characterization of Rat Embryonic Stem Cells In Vitro Maren Schulze, Hendrik Ungefroren, Michael Bader, and Fred Fändrich ........................................................................... 45 5 Derivation, Maintenance, and Induction of the Differentiation In Vitro of Equine Embryonic Stem Cells Shigeo Saito, Ken Sawai, Arika Minamihashi, Hideyo Ugai, Takehide Murata, and Kazunari K. Yokoyama ............................... 59 6 Generation and Characterization of Monkey Embryonic Stem Cells Hirofumi Suemori and Norio Nakatsuji ............................................. 81 7 Derivation and Propagation of Embryonic Stem Cells in Serum- and Feeder-Free Culture Jennifer Nichols and Qi-Long Ying ..................................................... 91 PART II. SIGNALING IN EMBRYONIC STEM CELL DIFFERENTIATION 8 Internal Standards in Differentiating Embryonic Stem Cells In Vitro Christopher L. Murphy...................................................................... 101 viii Contents 9 Matrix Assembly, Cell Polarization, and Cell Survival: Analysis of Peri-Implantation Development With Cultured Embryonic Stem Cells Shaohua Li and Peter D. Yurchenco ................................................. 113 10 Phosphoinositides, Inositol Phosphates, and Phospholipase C in Embryonic Stem Cells Leo R. Quinlan .................................................................................. 127 11 Cripto Signaling in Differentiating Embryonic Stem Cells Gabriella Minchiotti, Silvia Parisi, and M. Graziella Persico .............................................................. 151 12 The Use of Embryonic Stem Cells to Study Hedgehog Signaling Sandy Becker and Laura Grabel ....................................................... 171 13 Transfection and Promoter Analysis in Embryonic Stem Cells Sangmi Chung and Kwang-Soo Kim .................................................. 187 14 SAGE Analysis to Identify Embryonic Stem Cell-Predominant Transcripts Kenneth R. Boheler and Kirill V. Tarasov ......................................... 195 15 Utilization of Digital Differential Display to Identify Novel Targets of Oct3/4 Yoshimi Tokuzawa, Masayoshi Maruyama, and Shinya Yamanaka ................................................................... 223 16 Gene Silencing Using RNA Interference in Embryonic Stem Cells J. Matthew Velkey, Nicole A. Slawny, Theresa E. Gratsch, and K. Sue O’Shea ........................................................................ 233 PART III. GENETIC MANIPULATION OF EMBRYONIC STEM CELLS 17 Efficient Transfer of HSV-1 Amplicon Vectors Into Embryonic Stem Cells and Their Derivatives Dieter Riethmacher, Filip Lim, and Thomas Schimmang ................. 265 18 Lentiviral Vector-Mediated Gene Transfer in Embryonic Stem Cells Masahiro Oka, Lung-Ji Chang, Frank Costantini, and Naohiro Terada...................................................................... 273 19 Use of the Cytomegalovirus Promoter for Transient and Stable Transgene Expression in Mouse Embryonic Stem Cells Katie M. Barrow, Flor M. Perez-Campo, and Christopher M. Ward............................................................. 283 Contents ix 20 Use of Simian Immunodeficiency Virus Vectors for Simian Embryonic Stem Cells Takayuki Asano, Hiroaki Shibata, and Yutaka Hanazono ................ 295 21 Generation of Green Fluorescent Protein-Expressing Monkey Embryonic Stem Cells Tatsuyuki Takada, Yutaka Suzuki, Nae Kadota, Yasushi Kondo, and Ryuzo Torii................................................... 305 22 DNA Damage Response and Mutagenesis in Mouse Embryonic Stem Cells Yiling Hong, Rachel B. Cervantes, and Peter J. Stambrook ............. 313 23 Ultraviolet-Induced Apoptosis in Embryonic Stem Cells In Vitro Dakang Xu, Trevor J. Wilson, and Paul J. Hertzog ........................... 327 PART IV. USE OF EMBRYONIC STEM CELLS IN PHARMACOLOGICAL AND TOXICOLOGICAL SCREENS 24 Use of Differentiating Embryonic Stem Cells in Pharmacological Studies Brigitte Wdziekonski, Phi Villageois, Cécile Vernochet, Blaine Phillips, and Christian Dani............................................... 341 25 Embryonic Stem Cells as a Source of Differentiated Neural Cells for Pharmacological Screens Patrick J. Mee, Carmel M. O’Brien, Hazel Thomson, Sjaak van der Sar, Viktor Lakics, and Timothy E. Allsopp................................................................. 353 26 Use of Murine Embryonic Stem Cells in Embryotoxicity Assays: The Embryonic Stem Cell Test Andrea E. M. Seiler, Roland Buesen, Anke Visan, and Horst Spielmann .................................................................... 371 27 Use of Chemical Mutagenesis in Mouse Embryonic Stem Cells Sonja Becker, Martin Hrabé de Angelis, and Johannes Beckers ................................................................... 397 PART V. EPIGENETIC ANALYSIS OF EMBRYONIC STEM CELLS 28 Nuclear Reprogramming of Somatic Nucleus Hybridized With Embryonic Stem Cells by Electrofusion Masako Tada and Takashi Tada ........................................................ 411 29 Methylation in Embryonic Stem Cells In Vitro Koichiro Nishino, Jun Ohgane, Masako Suzuki, Naka Hattori, and Kunio Shiota ................................................... 421 PART VI. TUMOR-LIKE PROPERTIES 30 Identification of Genes Involved in Tumor-Like Properties of Embryonic Stem Cells Kazutoshi Takahashi, Tomoko Ichisaka, and Shinya Yamanaka ................................................................... 449 31 In Vivo Tumor Formation From Primate Embryonic Stem Cells Takayuki Asano, Kyoko Sasaki, Yoshihiro Kitano, Keiji Terao, and Yutaka Hanazono ............................................... 459 PART VII. ANIMAL MODELS AND THERAPY 32 Directed Differentiation and Characterization of Genetically Modified Embryonic Stem Cells for Therapy Adeline A. Lau, Kim M. Hemsley, Adrian Meedeniya, Aaron J. Robinson, and John J. Hopwood .................................... 471 33 Use of Differentiating Embryonic Stem Cells in the Parkinsonian Mouse Model Fumihiko Nishimura, Hayato Toriumi, Shigeaki Ishizaka, Toshisuke Sakaki, and Masahide Yoshikawa ................................ 485 Index ............................................................................................................ 495