Ion channels : methods and protocols
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : James D Stockand; Mark S Shapiro
- ناشر : Totowa, N.J. : Humana Press
- چاپ و سال / کشور: 2006
- شابک / ISBN : 9781597450959
Description
Functional reconstitution of the human epithelial Na+ channel in a mammalian expression system / Alexander Staruschenko ... [et al.] -- Overexpression of proteins in neurons using replication-deficient virus / Richard M. Ahlquist and Jane M. Sullivan -- Exogenous expression of proteins in neurons using the biolistic particle delivery system / Nikita Gamper and Mark S. Shapiro. Tertiary and quaternary structure formation of voltage-gated potassium channels / John M. Robinson, Andrey Kosolapov, and Carol Deutsch -- Biophysical approach to determine the subunit stoichiometry of the epithelial sodium channel using the xenopus laevis oocyte expression system / Farhad Kosari, Shaohu Sheng, and Thomas R. Kleyman -- Spectroscopy-based quantitative fluorescence resonance energy transfer analysis / Jie Zheng -- Probing the effects of phosphoinositides on ion channels / Chou-Long Huang -- Epithelial sodium channel in planar lipid bilayers / Bakhrom K. Berdiev and Dale J. Benos. A simple in vivo method for assessing changes of membrane-bound ion channel density in xenopus oocytes / Mouhamed S. Awayda ... [et al.] -- Preparation of cortical brain slices for electrophysiological recording / Costa M. Colbert -- Juxtacellular labeling and chemical phenotyping of extracellulary recorded neurons in vivo / Glenn M. Toney and Lynette C. Daws -- Carbon fiber amperometry in the study of ion channels and secretion / Duk-Su Koh -- Genetic screening for functionality of bacterial potassium channel mutants using K+ update-deficient escherichia coli / Lyubov V. Parfenova and Brad S. Rothberg -- KCNQ1 K+ channel-mediated cardiac channelopathies / Gildas Loussouarn, Isabelle Baró, and Denis Escande -- Tissue-specific transgenic and knockout mice / Andrée Porret ... [et al.].
Features diverse applications that range from the study of allosteric regulation of ion channel activity using a classic mutagenesis approach, to the study of channel subunit stoichiometry using a novel biophysical approach based on fluorescence resonance energy transfer.
