Alzheimer's disease and frontotemporal dementia : methods and protocols

Part I Working wi th AD- and FTD-Related Molecules 2 Preparing Synthetic Ab in Different Aggregation States . . . . . . . . . . . . . . . . . . . . 13 W. Blaine Stine, Lisa Jungbauer, Chunjiang Yu, and Mary Jo LaDu 3 Isolation of Low-n Amyloid b-Protein Oligomers from Cultured Cells, CSF, and Brain . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 Ganesh M. Shankar, Alfred T. Welzel, Jessica M. McDonald, Dennis J. Selkoe, and Dominic M. Walsh 4 Detecting Ab*56 Oligomers in Brain Tissues . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45 Mathew A. Sherman and Sylvain E. Lesné 5 Assessing Ab Aggregation State by Atomic Force Microscopy . . . . . . . . . . . . . . . 57 Justin Legleiter 6 Measuring APP Carboxy-Terminal Fragments . . . . . . . . . . . . . . . . . . . . . . . . . . . 71 Luke A. Esposito 7 Detection of APP Intracellular Domain in Brain Tissue . . . . . . . . . . . . . . . . . . . . 85 Sanjay W. Pimplikar and Anupama Suryanarayana 8 Cell-Based Assays for Regulators of Tau Biology . . . . . . . . . . . . . . . . . . . . . . . . . 93 Umesh K. Jinwal and Chad A. Dickey 9 Split GFP Complementation Assay for Quantitative Measurement of Tau Aggregation In Situ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109 Wanjoo Chun, Geoffrey S. Waldo, and Gail V.W. Johnson 10 Apolipoprotein E Expression and Purification . . . . . . . . . . . . . . . . . . . . . . . . . . . 125 Yvonne Newhouse and Karl H. Weisgraber Part II Model Systems and Outcome Measures 11 Ab Toxicity in Primary Cultured Neurons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 141 Adriana Ferreira, Roxana C. Sinjoanu, Alexandra Nicholson, and Sara Kleinschmidt 12 Manipulation of Gene Expression in the Central Nervous System with Lentiviral Vectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 155 Binggui Sun and Li Gan viii Contents 13 Selecting a Mouse Model of Alzheimer’s Disease . . . . . . . . . . . . . . . . . . . . . . . . . 169 Jeannie Chin 14 Monitoring Spatial Learning and Memory in Alzheimer’s Disease Mouse Models Using the Morris Water Maze . . . . . . . . . . . . . . . . . . . . . . . . . . . 191 Kimberly Scearce-Levie 15 Step-by-Step In Situ Hybridization Method for Localizing Gene Expression Changes in the Brain . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 207 Jorge J. Palop, Erik D. Roberson, and Inma Cobos 16 Real-Time Visualization of Axonal Transport in Neurons . . . . . . . . . . . . . . . . . . . 231 Yasuko Osakada and Bianxiao Cui 17 Quantifying Biomarkers of Cognitive Dysfunction and Neuronal Network Hyperexcitability in Mouse Models of Alzheimer’s Disease: Depletion of Calcium-Dependent Proteins and Inhibitory Hippocampal Remodeling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 245 Jorge J. Palop, Lennart Mucke, and Erik D. Roberson 18 Epigenetic Changes in the Brain: Measuring Global Histone Modifications . . . . . 263 Gavin Rumbaugh and Courtney A. Miller