Immunoelectron microscopy : methods and protocols

Protein antigen expression in escherichia coli for antibody production / D.M. Rancour, S.K. Backues and S.Y. Bednarek -- Expression of epitope-tagged proteins in plants / T. Furuichi -- Expression of epitope-tagged proteins in arabidopsis leaf mesophyll protoplasts / Y.H. Cho and S.D. Yoo -- Transient expression of epitope-tagged proteins in mammalian cells / M.L. Styers, J. Lowery and E. Sztul -- Production and purification of polyclonal antibodies / M. Nakazawa, M. Mukumoto and K. Miyatake -- Production and purification of monoclonal antibodies / M. Nakazawa, M. Mukumoto and K. Miyatake -- Production of antipeptide antibodies / B.S. Lee ... [et al.] -- Preparation of colloidal gold particles and conjugation to protein A, IgG, F(ab')(2), and streptavidin / S. Yokota -- Immunoelectron microscopy of chemically fixed developing plant embryos / T. Osafune and S.D. Schwartzbach -- Pre-embedding immunogold localization of antigens in mammalian brain slices / T. Schikorski -- Pre-embedding immunoelectron microscopy of chemically fixed mammalian tissue culture cells / H. Hagiwara ... [et al.] -- Immunoelectron microscopy of cryofixed and freeze-substituted plant tissues / M. Takeuchi, K. Takabe and Y. Mineyuki -- In vivo cryotechniques for preparation of animal tissues for immunoelectron microscopy / S. Ohno ... [et al.] -- Immunoelectron microscopy of cryofixed freeze-substituted mammalian tissue culture cells / A. Sawaguchi -- Immunoelectron microscopy of cryofixed freeze-substituted saccharomyces cerevisiae / J. Fiserova and M.W. Goldberg -- High-resolution molecular localization by freeze-fracture replica labeling / A. Fujita and T. Fujimoto -- Pre-embedding electron microscopy methods for glycan localization in chemically fixed mammalian tissue using horseradish peroxidase-conjugated lectin / Y. Akimoto and H. Kawakami -- Pre-embedding nanogold silver and gold intensification / A. Yamamoto and R. Masaki -- The post-embedding method for immunoelectron microscopy of mammalian tissues: A standardized procedure based on heat-induced antigen retrieval / S. Yamashita -- Double-label immunoelectron microscopy for studying the colocalization of proteins in cultured cells / H. Hagiwara ... [et al.] -- Serial section immunoelectron microscopy of algal cells / T. Osafune and S.D. Schwartzbach -- Freeze-etch electron tomography for the plasma membrane interface / N. Morone -- Localization of rDNA at nucleolar structural components by immunoelectron microscopy / S. Sato and Y. Sato -- Immunogold labelling for scanning electron microscopy / M.W. Goldberg and J. Fiserova -- Horseradish peroxidase as a reporter gene and as a cell-organelle-specific marker in correlative light-electron microscopy / T. Schikorski -- Monitoring rapid endocytosis in the electron microscope via photoconversion of vesicles fluorescently labeled with FM1-43 / T. Schikorski.