Potassium channels : methods and protocols

Contents: Identifying transcriptional regulatory regions using reporter genes and DNA-protein interactions by chromatin immunoprecipitation / Lezanne Ooi and Ian C. Wood -- Quantitative RT-PCR methods for investigation of low copy potassium channel gene expresssion in native murine arteries / Alex Cheong, Samuel J. Fountain, and David J. Beech -- Cloning of potassium chanel splice variants from tissues and cells / Lie Chen and Michael J. Shipston -- Chemiluminescence assays to investigate membrane expression and clathrin-mediated endocytosis of KATP channels / Andrew J. Smith and Asipu Sivaprasadarao -- Investigation of KATP channel endocytosis by immunofluorescence / Andrew J. Smith and Asipu Sivaprasadarao -- Investigation of KATP channel endocytosis and cell surface density by biotinylation and western blotting / Andrew J. Smith and Asipu Sivaprasadarao -- Lipid microdomains and K+ channel compartmentation : detergent and non-detergent-based methods for the isolation and characterisation of cholesterol-enriched lipid rafts / Laura J. Sampson and Caroline Dart -- Determination of phosphoinositide binding to K+ channel subunits using a protein-lipid overlay assay / Alison M. Thomas and Andrew Tinker -- Protein complex analysis of native brain potassium channels by proteomics / Guillaume Sandoz and Florian Lesage -- Xenopus oocytes as a heterologous expression system for studying ion channels with the patch-clamp technique / Paolo Tammaro, Kenju Shimomura, and Peter Proks -- Whole-cell recording using the perforated patch clamp technique / Jonathan D. Lippiat -- Recording the activity of ATP-sensitive K+ channels in open-cell cell-attached configuration / Andrei I. Tarasov -- Planar patch clamp : advances in electrophysiology / Andrea Brüggemann ... [et al.] -- Analysing steroid modulation of BKCa channels reconstituted into planar lipid bilayers / Heidi de Wet, Jonathan D. Lippiat, and Marcus Allen -- Using bioluminescence resonance energy transfer to measure ion channel assembly / Gina M. Whitaker and Eric A. Accili -- The use of FRET microscopy to elucidate steady state channel conformational rearrangements and G protein interaction with the GIRK channels / Adi Raveh, Inbal Riven, and Eitan Reuveny -- The voltage-clamp fluorometry technique / Chris S. Gandhi and Riccardo Olcese -- Identification of mutations in the Kir6.2 subunit of the KATP channel / Sarah E. Flanagan and Sian Ellard -- Modulation of potassium ion channel proteins utilising antibodies / Mark L. Dallas, Susan A. Deuchars, and Jim Deuchars -- Fluorescence-based Tl+-influx assays as a novel approach for characterization of small-conductance CA²⁺-activated K+ channel modulators / Susanne Jørgensen, Tina H. Johansen, and Tino Dyhring -- Rubidium efflux as a tool for the pharmacological characterisation of compounds with BK channel opening properties / Neil G. McKay, Robert W. Kirby, and Kim Lawson -- Recording hERG potassium currents and assessing the effects of compounds using the whole-cell patch-clamp technique / Ray M. Helliwell.