Cerebral glucose metabolism after traumatic brain injury in the rat studied by 13C-glucose and microdialysis
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Fredrik Clausen , Lars Hillered , Jan Gustafsson
- چاپ و سال / کشور: 2011
Description
Background Following traumatic brain injury (TBI), a disturbed cerebral glucose metabolism contributes to secondary brain damage. To study local cerebral glucose metabolism after TBI, we delivered 13C-labeled glucose into brain tissue by microdialysis (MD). Method MD probes were inserted bilaterally into the parietal cortex of rat brain, one probe in the shear stress zone of the injury and the other at the corresponding contralateral coordinates. A moderately severe controlled cortical contusion was used to model TBI. Dialysate concentrations of glucose, pyruvate, lactate, and glycerol were measured, and following derivatization, 13C enrichments of the compounds were determined by gas chromatography–mass spectrometry. Findings We found that 13C-labeled glucose was rapidly converted into 13C-lactate and 13C-glycerol. In the hours following TBI, concentrations and 13C enrichments of lactate and glycerol increased. Conclusions The findings confirm the occurrence of anaerobic local glucose metabolism early after TBI. Only a small fraction of the glycerol was newly synthesized, suggesting that the hypothesis that most of the released glycerol after TBI comes from degradation of membrane phospholipids still holds.We conclude that the combination of microdialysis and stable isotope technique is a useful tool for investigating local glucose metabolism following brain injury.
Acta Neurochir (2011) 153:653–658 DOI 10.1007/s00701-010-0871-7 Received: 13 September 2010 / Accepted: 3 November 2010 / Published online: 21 November 2010 # Springer-Verlag 2010
