Non-promoter hypermethylation of zygote arrest 1 (ZAR1) in human brain tumors
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Takao Watanabe Kazunari Yachi Takashi Ohta Takao Fukushima Atsuo Yoshino Yoichi Katayama Yui Shinojima Tadashi Terui Hiroki Nagase
- چاپ و سال / کشور: 2011
Description
Zygote arrest 1 (ZAR1) is a novel maternal-effect gene of crucial importance during the oocyte-to-embryo transition. Comprehensive methylation analysis of tumorspecific differently methylated regions in human malignant melanomas has recently led to the identification of nonpromoter hypermethylation of the ZAR1 gene that had never been identified as an aberrant methylated region in any human tumor. Notably, ZAR1 hypermethylation was frequently observed in melanomas but was absent in benign nevi, and ZAR1 expression was found to be up-regulated in methylated tumors. These findings prompted us to screen for ZAR1 non-promoter methylation in various types of human brain tumors using MassARRAY EpiTYPER. Strikingly, hypermethylation of ZAR1 was observed frequently in diffuse astrocytomas (100%), anaplastic astrocytomas (94%), glioblastomas (93%), oligodendrogliomas (100%), anaplastic oligodendrogliomas (100%), and pituitary adenomas (90%), but not at all in pilocytic astrocytomas. For other tumor types ZAR1 hypermethylation was infrequent: 17% of vestibular schwannomas and 33% of meningothelial meningiomas. Detectable ZAR1 transcript was not found in any of hypermethylated glioma cell lines. Our results indicate that hypermethylation of the ZAR1 non-promoter is extremely frequent in diffuse gliomas and pituitary adenomas, although ZAR1 expression is unlikely to play a tumorigenic role.
Brain Tumor Pathol (2011) 28:199–202 DOI 10.1007/s10014-011-0019-3Received: 25 November 2010 / Accepted: 8 January 2011 / Published online: 18 February 2011 The Japan Society of Brain Tumor Pathology 2011
