A miR-21 inhibitor enhances apoptosis and reduces G2-M accumulation induced by ionizing radiation in human glioblastoma U251 cells
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Yi Li Shiguang Zhao Yunbo Zhen Qiang Li Lei Teng Akio Asai Keiji Kawamot
- چاپ و سال / کشور: 2011
Description
MicroRNAs (miRNAs) are small noncoding RNAs that take part in diverse biological processes by suppressing target gene expression. Elevated expression of miR-21 has been reported in many types of human cancers. Radiotherapy is a standard adjuvant treatment for patients with glioblastoma. However, the resistance of glioblastoma cells to radiation limits the success of this treatment. In this study, we found that miR-21 expression was upregulated in response to ionizing radiation (IR) in U251 cells, which suggested that miR-21 could be involved in the response of U251 cells to radiation. We showed that a miR-21 inhibitor enhanced IR-induced glioblastoma cell growth arrest and increased the level of apoptosis, which was probably caused by abrogation of the G2-M arrest induced by IR. Further research demonstrated that the miR-21 inhibitor induced the upregulation of Cdc25A. Taken together, these findings suggest that miR-21 inhibitor can increase IRinduced growth arrest and apoptosis in U251 glioblastoma cells, at least in part by abrogating G2-M arrest, and that Cdc25A is a potential target of miR-21.
Brain Tumor Pathol (2011) 28:209–214 DOI 10.1007/s10014-011-0037-1 Received: 9 February 2011 / Accepted: 20 April 2011 / Published online: 27 May 2011 The Japan Society of Brain Tumor Pathology 2011
