Gene expression array testing of FFPE archival breast tumor samples: an optimized protocol for WG-DASL sample preparation
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : C. C. Ton N. Vartanian X. Chai M. G. Lin X. Yuan K. E. Malone C. I. Li A. Dawson C. Sather J. Delrow L. Hsu P. L. Porter
- چاپ و سال / کشور: 2010
Description
Archived formalin-fixed, paraffin embedded (FFPE) tissues constitute a vast, well-annotated, but underexploited resource for the molecular study of cancer progression, largely because degradation, chemical modification, and cross-linking, render FFPE RNA a suboptimal substrate for conventional analytical methods. We report here a modified protocol for RNA extraction from FFPE tissues which maximized the success rate (with 100% of samples) in the expression profiling of a set of 60 breast cancer samples on the WG-DASL platform; yielding data of sufficient quality such that in hierarchical clustering (a) 12/12 (100%) replicates correctly identified their respective counterparts, with a high self-correlation (r = 0.979), and (b) the overall sample set grouped with high specificity into ER? (38/40; 95%) and ER- (18/20; 90%) subtypes. These results indicate that a large fraction of decade-old FFPE samples, of diverse institutional origins and processing histories, can yield RNA suitable for gene expression profiling experiments.
Breast Cancer Res Treat (2011) 125:879–883 DOI 10.1007/s10549-010-1159-6 Received: 19 August 2010 / Accepted: 31 August 2010 / Published online: 15 September 2010
