CAML promotes prolactin-dependent proliferation of breast cancer cells by facilitating prolactin receptor signaling pathways
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Ji-Hong Lim Tae-You Kim Woo-Ho Kim Jong-Wan Park
- چاپ و سال / کشور: 2010
Description
Calcium-modulating cyclophilin ligand (CAML) interacts with the intracellular regions of various types of membrane-bound receptors, and is required for signaling pathways that involve these receptors. The authors tested the possibility that CAML interacts with prolactin receptor (PRLR) and participates in the prolactin (PRL)-dependent proliferation of breast cancer cells. Breast cancer cell lines were found to express CAML at higher levels than cell lines originating from leukemia and other cancers. Furthermore, immunohistochemical analyses of breast tissue arrays showed that the CAML levels were higher in cancer tissues than in normal tissues. In breast cancer cells and transfected HEK293 cells, CAML associated with PRLR, and this interaction was augmented during PRL stimulation. CAMLsilencing experiments revealed that CAML is required for the functions of PRLR, such as, the activations of Stat5 and Mek1/2, PRL internalization with cyclophilin B, PRLR recycling, and increased Ca2? mobilization. In addition, CAML was found to play a crucial role in the PRL/PRLRdependent growth of breast cancer cells. These results suggest a new role for CAML in breast cancer development.
DOI 10.1007/s10549-010-1274-4 Received: 3 September 2010 / Accepted: 17 November 2010
