The effect of vitamin C on amiodarone-induced toxicity in rat thymocytes
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Cekic S1, Pavlovic D2,Sarac M3, Kamenov B4, Dimic A5, Pavlovic V1
- چاپ و سال / کشور: 2010
Description
Although, the antiarrhythmic effect of amiodarone (AMD) is well characterized, the mechanism of its toxicity on extracardiac tissues is still poorly understood. Several antioxidants have been shown to prevent AMD-induced toxicity by antioxidant and/or non-antioxidant mechanisms. In the current study, we evaluated the possible protective effect, in vitro, of vitamin C on AMD-induced toxicity in rat thymocytes. Rat thymocytes were cultured with increasing AMD concentrations (1–20 μM) with or without vitamin C (1000 μg/ml), for 24 hours. Cells treatment with AMD resulted in a concentration-dependent increase of hypodiploid cells and a significant decrease in cellular glutathione content. Vitamin C combined with AMD significantly decreased the proportion of hypodiploid cells and markedly increased the cellular glutathione content, compared with AMD treatment alone. These results suggest that treatment with vitamin C may prevent AMD-induced toxicity in rat thymocytes by restoring cellular glutathione content.
Cent. Eur. J. Med. , 6(1) , 2010 , 58-63 Received 19 April 2010; Accepted 5 August 2010
