The histone deacetylase inhibitor sodium butyrate in combination with brain-derived neurotrophic factor reduces the viability of DAOY human medulloblastoma cells
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Carolina Nör & Caroline Brunetto de Farias & Ana Lucia Abujamra & Gilberto Schwartsmann & Algemir Lunardi Brunetto & Rafael Roesler
- چاپ و سال / کشور: 2011
Description
Purpose Histone deacetylase inhibitors (HDACis) are a promising class of anticancer agents for the treatment of brain tumors. HDACis can increase the expression of brainderived neurotrophic factor (BDNF) in brain cells. We have previously shown that BDNF reduces the viability of medulloblastoma cells. The aim of the present study was to examine the effect of the HDACi sodium butyrate (NaB) combined with human recombinant BDNF (hrBDNF), on the viability of human medulloblastoma cell lines. Methods DAOY and ONS76 medulloblastoma cells were treated with NaB, hrBDNF, or NaB combined with hrBDNF. Cell viability was measured with the MTT assay. Results NaB combined with hrBDNF significantly reduced the viability of DAOY medulloblastoma cells. In ONS76 cells, NaB alone reduced viability, but the effect was not potentiated by hrBDNF. Conclusion These findings provide early evidence for a rationale supporting further evaluation of HDACis and BDNF as a new combinatorial approach to inhibit the growth of medulloblastoma
Childs Nerv Syst (2011) 27:897–901 DOI 10.1007/s00381-011-1439-4 Received: 16 February 2011 / Accepted: 17 March 2011 / Published online: 6 April 2011
