b1,4-galactosyltransferase-I in synovial tissue of collagen-induced rat model of rheumatoid arthritis
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Hairong Wang Dawei Xu Ran Tao Xiaohui Ni Aiguo Shen Youhua Wang
- چاپ و سال / کشور: 2010
Description
b1,4-galactosyltransferase-I (b1,4-GalT-I), which is one of the best-studied glycosyltransferases, plays a key role in the synthesis of selectin ligands such as sialyl Lewis (sLex) and sulfated sLex. Previous studies showed that inflammatory responses of b1,4-GalT-I-deficient mice were impaired because of the defect in selectin-ligand biosynthesis. However, the expression of b1,4-GalT-I and its biological function in rheumatoid arthritis (RA) remain to be elucidated. The mRNA and protein expression of b1,4-GalT-I increased in synovial tissue of the RA group compared with the Normal group at 10d and 15d after collagen-induced. Double staining indicated b1,4-GalT-I overlapped with macrophage-like synoviocytes (MLSs), fibroblast-like synoviocytes (FLSs), neutrophils and tumor necrosis factor-a (TNF-a). Moreover, b1,4-GalT-I mRNA in FLSs in vitro was affected in a dose- and time-dependent manner in response to TNF-a stimulation. ELISA revealed that expression of TNF-a was attenuated in FLSs in vitro treated with b1,4-GalT-I-Ab. We therefore suggest that b1,4-GalT-I may play an important role in the inflammation process of RA synovial tissue, which would provide the foundation for further researching into the concrete mechanism of b1,4-GalT-I in RA
Received: 2 August 2010 / Accepted: 29 October 2010 / Published online: 16 December 2010 Clin Exp Med (2011) 11:147–154 DOI 10.1007/s10238-010-0121-6
