Anti-inflammatory effects of EMD in the presence of biomechanical loading and interleukin-1â in vitro
- نوع فایل : کتاب
- زبان : انگلیسی
- مؤلف : Marjan Nokhbehsaim & Birgit Deschner & Jochen Winter & Christoph Bourauel & Andreas Jنger & Sّren Jepsen & James Deschner
- چاپ و سال / کشور: 2010
Description
Enamel matrix derivative (EMD) used to promote periodontal regeneration has been shown to exert antiinflammatory effects. This in vitro study was performed to investigate if the anti-inflammatory actions of EMD are modulated by the local cellular environment, such as inflammation or occlusal, i.e., biomechanical, loading. Human periodontal ligament cells were seeded on BioFlex plates and incubated with EMD under normal, inflammatory, and biomechanical loading conditions for 1 and 6 days. In order to mimic inflammatory and biomechanical loading conditions in vitro, cells were stimulated with interleukin (IL)-1â and exposed to dynamic tensile strain, respectively. The gene expression of IL-1â, IL-1 receptor antagonist (IL-1RN), IL-6, IL-8, IL-10, and cyclooxygenase (COX)-2 was analyzed by real-time RT-PCR and the IL-6 protein synthesis by enzyme-linked immunoassay. For statistical analysis, Student's t test, ANOVA, and post-hoc comparison tests were applied (p<0.05). EMD downregulated significantly the expression of IL-1â and COX-2 at 1 day and of IL-6, IL-8, and COX-2 at 6 days in normal condition. In an inflammatory environment, the antiinflammatory actions of EMD were significantly enhanced at 6 days. In the presence of low biomechanical loading, EMD caused a downregulation of IL-1â and IL-8, whereas high biomechanical loading significantly abrogated the anti-inflammatory effects of EMD at both days. Neither IL-1RN nor IL-10 was upregulated by EMD. These data suggest that high occlusal forces may abrogate antiinflammatory effects of EMD and should, therefore, be avoided immediately after the application of EMD to achieve best healing results.
Clin Oral Invest DOI 10.1007/s00784-010-0505-8 Received: 21 September 2010 / Accepted: 30 December 2010
